The transition of zebrafish functional genetics from random mutagenesis to targeted integration

Louis Y.El Khoury, Jarryd M. Campbell, Karl J. Clark

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The zebrafish is a popular animal model for the study of various aspects of health and disease due to its high fecundity, rapid embryonic development, relatively low maintenance costs, and increasing genetic malleability. In light of the high genetic tractability of zebrafish and the constant developments observed in the field of genetic engineering, this chapter reviews some of the major random and targeted gene-editing methods employed in the generation of a variety of mutant and transgenic fish lines to better understand genotype-phenotype relationships. Furthermore, this chapter proposes a novel zebrafish larvae mutagenesis and genotyping approach by coupling targeted integrations with mutagenic gene-break transposon vectors containing fluorescent reporters.

Original languageEnglish (US)
Title of host publicationMolecular-Genetic and Statistical Techniques for Behavioral and Neural Research
PublisherElsevier
Pages401-416
Number of pages16
ISBN (Electronic)9780128040782
ISBN (Print)9780128041161
DOIs
StatePublished - Jan 1 2018

Keywords

  • CRISPR
  • Gene editing
  • Gene traps
  • Gene-break transposons
  • Genetic engineering
  • Homology-directed repair (HDR)
  • Nonhomologous end joining (NHEJ)
  • TALEN
  • Zebrafish
  • ZFN

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

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