The therapeutic window of hypothermic neuroprotection in experimental ischemic neuropathy: Protection in ischemic phase and potential deterioration in later reperfusion phase

Nobutoshi Kawamura, James D. Schmelzer, Yanping Wang, Ann M. Schmeichel, Phillip Anson Low

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hypothermia will neuroprotect peripheral nerve from ischemia-reperfusion (IR) injury, but the therapeutic window of hypothermic neuroprotection has not been defined. Unilateral IR injury was produced by the ligation and release of nooses tied around supplying arteries to the right sciatic-tibial nerve of the rat. Using this model, 114 rats were divided into 12 groups according to the delay (0, 1, 3, and 4 h) and the depth of hypothermia (28, 32, and 35°C). All rats were subjected to 3 h ischemia and 7 days reperfusion followed by behavioral, electrophysiological, and pathological evaluations. We demonstrated significant hypothermic neuroprotection with both deep (28°C) and mild (32°C) hypothermia initiated during ischemia (0 and 1 h delay), but not hypothermia initiated during reperfusion (3 and 4 h delay) in both behavioral and electrophysiological evaluations. In addition, the pathologically significant differences were observed between deep hypothermia (28°C) and normothermia (35°C) initiated during ischemia. We conclude that the therapeutic window of hypothermic neuroprotection is optimal during the intraischemic period and that mild and deep hypothermia provide neuroprotection. Prolonged delay of hypothermic treatment results in worsening of IR injury.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalExperimental Neurology
Volume195
Issue number2
DOIs
StatePublished - Oct 2005

Fingerprint

Hypothermia
Reperfusion
Reperfusion Injury
Ischemia
Therapeutics
Tibial Nerve
Sciatic Nerve
Peripheral Nerves
Ligation
Neuroprotection
Arteries

Keywords

  • Hypothermia
  • Ischemic neuropathy
  • Neuroprotection
  • Reperfusion
  • Therapeutic window

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

The therapeutic window of hypothermic neuroprotection in experimental ischemic neuropathy : Protection in ischemic phase and potential deterioration in later reperfusion phase. / Kawamura, Nobutoshi; Schmelzer, James D.; Wang, Yanping; Schmeichel, Ann M.; Low, Phillip Anson.

In: Experimental Neurology, Vol. 195, No. 2, 10.2005, p. 305-312.

Research output: Contribution to journalArticle

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