TY - JOUR
T1 - The synovial production of collagenase and chondrocyte activating factors in response to cobalt
AU - Ferguson, Gary M.
AU - Watanabe, Shoji
AU - Georgescu, Helga I.
AU - Evans, Christopher H.
PY - 1988/7
Y1 - 1988/7
N2 - Addition of CoCl2 solutions to the culture media of confluent monolayers of lapine or human synoviocytes stimulated their production of the neutral proteinases collagenase, gelatinase, and caseinase. With lapine cells, maximum stimulation occurred at 10−7 M CoCl2, while human cells required 10−4–10−5 M CoCl2 to achieve a maximum stimulation. Production of prostaglandin E2 by lapine cells was enhanced some 30–40% by concentrations of CoCl2 that maximally stimulated synthesis of the neutral proteinases, whereas all concentrations of CoCl2 slightly depressed the productio of prostaglandin E2 by human celis. Lapine synovial cells that had been stimulated by CoCl2 also produced a substance, or substances, that provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by monolayers of articular chondrocytes. Chondrocytes themselves, however, resisted activation by CoCl2. These findings may be relevant to the aseptic loosening of joint prostheses.
AB - Addition of CoCl2 solutions to the culture media of confluent monolayers of lapine or human synoviocytes stimulated their production of the neutral proteinases collagenase, gelatinase, and caseinase. With lapine cells, maximum stimulation occurred at 10−7 M CoCl2, while human cells required 10−4–10−5 M CoCl2 to achieve a maximum stimulation. Production of prostaglandin E2 by lapine cells was enhanced some 30–40% by concentrations of CoCl2 that maximally stimulated synthesis of the neutral proteinases, whereas all concentrations of CoCl2 slightly depressed the productio of prostaglandin E2 by human celis. Lapine synovial cells that had been stimulated by CoCl2 also produced a substance, or substances, that provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E2 by monolayers of articular chondrocytes. Chondrocytes themselves, however, resisted activation by CoCl2. These findings may be relevant to the aseptic loosening of joint prostheses.
KW - Cobalt
KW - Collagenase
KW - Prosthesis
KW - Synoviocyte
KW - loosening
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U2 - 10.1002/jor.1100060408
DO - 10.1002/jor.1100060408
M3 - Article
C2 - 2837555
AN - SCOPUS:0024053941
SN - 0736-0266
VL - 6
SP - 525
EP - 530
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 4
ER -