TY - CHAP
T1 - The Study of Brain Tumor Stem Cell Migration
AU - Lara-Velazquez, Montserrat
AU - Al-Kharboosh, Rawan
AU - Prieto, Luis
AU - Schiapparelli, Paula
AU - Quiñones-Hinojosa, Alfredo
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Despite many advancements in brain cancer therapeutics, brain cancer remains one of the most elusive diseases with high migratory capacity and a dismal prognosis. It is well established that tumor stem cells utilize the same available migratory machinery that normal cells employ. Some of the major determinants of brain tumor stem cell migration are their cytoskeletal rearrangements and adhesion dynamics. This phenomenon allows brain tumor stem cells to perpetually migrate, invade, and repopulate in a vicious cycle leading to tumor expansion and invasion at tumor boundaries. In order to dissect the enabling factors that allow for this process to be hijacked, we have identified relevant assays to enable measurements of neoplastic migration such as Boyden Chamber, 3D chemogradient chamber, Nanopattern, and wound healing assays. Our purpose is to report the complex experimental platforms seen in the literature today and provide an optimal platform to kick off your studies in this field.
AB - Despite many advancements in brain cancer therapeutics, brain cancer remains one of the most elusive diseases with high migratory capacity and a dismal prognosis. It is well established that tumor stem cells utilize the same available migratory machinery that normal cells employ. Some of the major determinants of brain tumor stem cell migration are their cytoskeletal rearrangements and adhesion dynamics. This phenomenon allows brain tumor stem cells to perpetually migrate, invade, and repopulate in a vicious cycle leading to tumor expansion and invasion at tumor boundaries. In order to dissect the enabling factors that allow for this process to be hijacked, we have identified relevant assays to enable measurements of neoplastic migration such as Boyden Chamber, 3D chemogradient chamber, Nanopattern, and wound healing assays. Our purpose is to report the complex experimental platforms seen in the literature today and provide an optimal platform to kick off your studies in this field.
KW - BTICs migration
KW - Boyden chamber
KW - Chemogradient chamber
KW - Nanopattern
KW - Transwell
KW - Wound healing assay
UR - http://www.scopus.com/inward/record.url?scp=85054890418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054890418&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8805-1_9
DO - 10.1007/978-1-4939-8805-1_9
M3 - Chapter
C2 - 30324517
AN - SCOPUS:85054890418
T3 - Methods in Molecular Biology
SP - 93
EP - 104
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -