The Study of Brain Tumor Stem Cell Migration

Montserrat Lara-Velazquez, Rawan Al-Kharboosh, Luis Prieto, Paula Schiapparelli, Alfredo Quinones-Hinojosa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Despite many advancements in brain cancer therapeutics, brain cancer remains one of the most elusive diseases with high migratory capacity and a dismal prognosis. It is well established that tumor stem cells utilize the same available migratory machinery that normal cells employ. Some of the major determinants of brain tumor stem cell migration are their cytoskeletal rearrangements and adhesion dynamics. This phenomenon allows brain tumor stem cells to perpetually migrate, invade, and repopulate in a vicious cycle leading to tumor expansion and invasion at tumor boundaries. In order to dissect the enabling factors that allow for this process to be hijacked, we have identified relevant assays to enable measurements of neoplastic migration such as Boyden Chamber, 3D chemogradient chamber, Nanopattern, and wound healing assays. Our purpose is to report the complex experimental platforms seen in the literature today and provide an optimal platform to kick off your studies in this field.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages93-104
Number of pages12
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1869
ISSN (Print)1064-3745

Fingerprint

Neoplastic Stem Cells
Brain Neoplasms
Cell Movement
Causality
Wound Healing
Neoplasms

Keywords

  • Boyden chamber
  • BTICs migration
  • Chemogradient chamber
  • Nanopattern
  • Transwell
  • Wound healing assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lara-Velazquez, M., Al-Kharboosh, R., Prieto, L., Schiapparelli, P., & Quinones-Hinojosa, A. (2019). The Study of Brain Tumor Stem Cell Migration. In Methods in Molecular Biology (pp. 93-104). (Methods in Molecular Biology; Vol. 1869). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8805-1_9

The Study of Brain Tumor Stem Cell Migration. / Lara-Velazquez, Montserrat; Al-Kharboosh, Rawan; Prieto, Luis; Schiapparelli, Paula; Quinones-Hinojosa, Alfredo.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 93-104 (Methods in Molecular Biology; Vol. 1869).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lara-Velazquez, M, Al-Kharboosh, R, Prieto, L, Schiapparelli, P & Quinones-Hinojosa, A 2019, The Study of Brain Tumor Stem Cell Migration. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1869, Humana Press Inc., pp. 93-104. https://doi.org/10.1007/978-1-4939-8805-1_9
Lara-Velazquez M, Al-Kharboosh R, Prieto L, Schiapparelli P, Quinones-Hinojosa A. The Study of Brain Tumor Stem Cell Migration. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 93-104. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8805-1_9
Lara-Velazquez, Montserrat ; Al-Kharboosh, Rawan ; Prieto, Luis ; Schiapparelli, Paula ; Quinones-Hinojosa, Alfredo. / The Study of Brain Tumor Stem Cell Migration. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 93-104 (Methods in Molecular Biology).
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