The Stability of Solubilized Mammalian Muscle Acetylcholine Receptors During Purification by Monoclonal Immunoadsorption

Mariko Yoshida Momoi, Vanda A. Lennon

Research output: Contribution to journalArticle

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Abstract

Abstract: The stability of nicotinic acetylcholine receptors (AChR) solubilized from mammalian skeletal muscle in nonionic detergent was investigated under various conditions of pH, chaotropic ions, and unfolding reagents in order to allow its purification in high yield by immunoadsorption to monoclonal antibodies. Preservation of the antigenicity and/or binding sites for α‐bungarotoxin was used as an indicator of the receptor protein's integrity. Both were preserved in the pH range 6.5–8.0, but when exposed for 1 h at 4°C to a pH outside this range, >50% activity was lost. Of the chaotropic ions studied (NaSCN, Nal, NaNO3, NaCl), only NaCl was tolerated. Most of the AChR's toxin‐binding activity was preserved after ex posure to 2 M NaCl, which was suitable for dissociating AChR when a monoclonal antibody with relatively low binding affinity was selected as the immunoadsorbent. Yields of purified AChR were optimal (30%) when a low amount of monoclonal antibody was coupled to cyanogen bromide‐activated agarose (1 mg protein/ml gel).

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalJournal of neurochemistry
Volume42
Issue number1
DOIs
StatePublished - Jan 1984

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Keywords

  • Acetylcholine receptors
  • Conditions
  • Mammalian skeletal muscle
  • Monoclonal immunoadsorption
  • Purification

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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