The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene

Eric G. Marcusson, Bruce F Horazdovsky, Joan Lin Cereghino, Editte Gharakhanian, Scott D. Emr

Research output: Contribution to journalArticle

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Abstract

The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.

Original languageEnglish (US)
Pages (from-to)579-586
Number of pages8
JournalCell
Volume77
Issue number4
DOIs
StatePublished - May 20 1994
Externally publishedYes

Fingerprint

Cathepsin A
Sorting
Yeast
Genes
Yeasts
Proteins
Amino Acids
beta-Fructofuranosidase
Endosomes
Protein Transport
Fractionation
Vacuoles
Mutation
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Marcusson, E. G., Horazdovsky, B. F., Cereghino, J. L., Gharakhanian, E., & Emr, S. D. (1994). The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell, 77(4), 579-586. https://doi.org/10.1016/0092-8674(94)90219-4

The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. / Marcusson, Eric G.; Horazdovsky, Bruce F; Cereghino, Joan Lin; Gharakhanian, Editte; Emr, Scott D.

In: Cell, Vol. 77, No. 4, 20.05.1994, p. 579-586.

Research output: Contribution to journalArticle

Marcusson, EG, Horazdovsky, BF, Cereghino, JL, Gharakhanian, E & Emr, SD 1994, 'The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene', Cell, vol. 77, no. 4, pp. 579-586. https://doi.org/10.1016/0092-8674(94)90219-4
Marcusson EG, Horazdovsky BF, Cereghino JL, Gharakhanian E, Emr SD. The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell. 1994 May 20;77(4):579-586. https://doi.org/10.1016/0092-8674(94)90219-4
Marcusson, Eric G. ; Horazdovsky, Bruce F ; Cereghino, Joan Lin ; Gharakhanian, Editte ; Emr, Scott D. / The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. In: Cell. 1994 ; Vol. 77, No. 4. pp. 579-586.
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