The sodium iodide symporter (NIS) as an imaging reporter for gene, viral, and cell-based therapies

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Preclinical and clinical tomographic imaging systems increasingly are being utilized for non-invasive imaging of reporter gene products to reveal the distribution of molecular therapeutics within living subjects. Reporter gene and probe combinations can be employed to monitor vectors for gene, viral, and cell-based therapies. There are several reporter systems available; however, those employing radionuclides for positron emission tomography (PET) or single-photon emission computed tomography (SPECT) offer the highest sensitivity and the greatest promise for deep tissue imaging in humans. Within the category of radionuclide reporters, the thyroidal sodium iodide symporter (NIS) has emerged as one of the most promising for preclinical and translational research. NIS has been incorporated into a remarkable variety of viral and non-viral vectors in which its functionality is conveniently determined by in vitro iodide uptake assays prior to live animal imaging. This review on the NIS reporter will focus on 1) differences between endogenous NIS and heterologously-expressed NIS, 2) qualitative or comparative use of NIS as an imaging reporter in preclinical and translational gene therapy, oncolytic viral therapy, and cell trafficking research, and 3) use of NIS as an absolute quantitative reporter.

Original languageEnglish (US)
Pages (from-to)33-47
Number of pages15
JournalCurrent Gene Therapy
Volume12
Issue number1
StatePublished - Feb 2012

Fingerprint

Cell- and Tissue-Based Therapy
Reporter Genes
Radioisotopes
Translational Medical Research
Viral Genes
Iodides
Single-Photon Emission-Computed Tomography
Genetic Therapy
Positron-Emission Tomography
Research
sodium-iodide symporter
Therapeutics
In Vitro Techniques

Keywords

  • Gene therapy
  • Imaging
  • NIS
  • Oncolytic virus
  • PET
  • Reporter gene
  • Sodium iodide symporter
  • SPECT

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)
  • Drug Discovery

Cite this

The sodium iodide symporter (NIS) as an imaging reporter for gene, viral, and cell-based therapies. / Penheiter, Alan R.; Russell, Stephen J; Carlson, Stephanie K.

In: Current Gene Therapy, Vol. 12, No. 1, 02.2012, p. 33-47.

Research output: Contribution to journalArticle

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