The small nuclear RNAs of Drosophila

Evelyne Myslinski, Christiane Branlant, Eric D Wieben, Thoru Pederson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have investigated the sequences of the major small nuclear RNAs of Drosophila cultured cells, with the objective of elucidating phylogenetically conserved primary and secondary structures by comparison of the data with previously determined sequences of these RNAs in vertebrate species. Our results reveal striking degrees of conservation between each Drosophila RNA and its vertebrate cognate, and also demonstrate blocks of homology among the Drosophila small nuclear RNAs, as previously described for vertebrates. The most conserved features include the 5′ terminal region of U1 RNA, thought to function in pre-mRNA splicing, most of the regions of U4 RNA recently implicated in 3′ processing of pre-mRNA, and the major snRNP protein binding site ("domain A") that is also shared by vertebrate U1, U2, U4 and U5 RNAs. Several other conserved features have been revealed, suggesting additional regions of functional significance in these RNAs and also providing further insights into the evolutionary history of the small nuclear RNAs.

Original languageEnglish (US)
Pages (from-to)927-945
Number of pages19
JournalJournal of Molecular Biology
Volume180
Issue number4
DOIs
StatePublished - Dec 25 1984
Externally publishedYes

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Small Nuclear RNA
Drosophila
Vertebrates
RNA Precursors
RNA
Small Nuclear Ribonucleoproteins
Protein Binding
Cultured Cells
History
Binding Sites
U4 small nuclear RNA
U1 small nuclear RNA

ASJC Scopus subject areas

  • Virology

Cite this

The small nuclear RNAs of Drosophila. / Myslinski, Evelyne; Branlant, Christiane; Wieben, Eric D; Pederson, Thoru.

In: Journal of Molecular Biology, Vol. 180, No. 4, 25.12.1984, p. 927-945.

Research output: Contribution to journalArticle

Myslinski, E, Branlant, C, Wieben, ED & Pederson, T 1984, 'The small nuclear RNAs of Drosophila', Journal of Molecular Biology, vol. 180, no. 4, pp. 927-945. https://doi.org/10.1016/0022-2836(84)90264-X
Myslinski, Evelyne ; Branlant, Christiane ; Wieben, Eric D ; Pederson, Thoru. / The small nuclear RNAs of Drosophila. In: Journal of Molecular Biology. 1984 ; Vol. 180, No. 4. pp. 927-945.
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