TY - JOUR
T1 - The small nuclear RNAs of Drosophila
AU - Myslinski, Evelyne
AU - Branlant, Christiane
AU - Wieben, Eric D.
AU - Pederson, Thoru
N1 - Funding Information:
This work was supported by grants to C.B. from the Centre National de la Recherchr Scientifique and to T.P. from the N.I.H. (GM28274). E.D.W. was the recipient of a N.I.H. postdoctoral fellowship (CA-06751). We thank F. Eyermann (Strasbourg) for excellent technical assistance, Joan Nenninger (Shrewsbury)f or maintaining and preparing the Sm monoclonal antibody and Jacqueline Foss (Shrewsbury) for typing the drafts and manuscript.
PY - 1984/12/25
Y1 - 1984/12/25
N2 - We have investigated the sequences of the major small nuclear RNAs of Drosophila cultured cells, with the objective of elucidating phylogenetically conserved primary and secondary structures by comparison of the data with previously determined sequences of these RNAs in vertebrate species. Our results reveal striking degrees of conservation between each Drosophila RNA and its vertebrate cognate, and also demonstrate blocks of homology among the Drosophila small nuclear RNAs, as previously described for vertebrates. The most conserved features include the 5′ terminal region of U1 RNA, thought to function in pre-mRNA splicing, most of the regions of U4 RNA recently implicated in 3′ processing of pre-mRNA, and the major snRNP protein binding site ("domain A") that is also shared by vertebrate U1, U2, U4 and U5 RNAs. Several other conserved features have been revealed, suggesting additional regions of functional significance in these RNAs and also providing further insights into the evolutionary history of the small nuclear RNAs.
AB - We have investigated the sequences of the major small nuclear RNAs of Drosophila cultured cells, with the objective of elucidating phylogenetically conserved primary and secondary structures by comparison of the data with previously determined sequences of these RNAs in vertebrate species. Our results reveal striking degrees of conservation between each Drosophila RNA and its vertebrate cognate, and also demonstrate blocks of homology among the Drosophila small nuclear RNAs, as previously described for vertebrates. The most conserved features include the 5′ terminal region of U1 RNA, thought to function in pre-mRNA splicing, most of the regions of U4 RNA recently implicated in 3′ processing of pre-mRNA, and the major snRNP protein binding site ("domain A") that is also shared by vertebrate U1, U2, U4 and U5 RNAs. Several other conserved features have been revealed, suggesting additional regions of functional significance in these RNAs and also providing further insights into the evolutionary history of the small nuclear RNAs.
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U2 - 10.1016/0022-2836(84)90264-X
DO - 10.1016/0022-2836(84)90264-X
M3 - Article
C2 - 6084723
AN - SCOPUS:0021631080
SN - 0022-2836
VL - 180
SP - 927
EP - 945
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -