The small GTPases ARL-13 and ARL-3 coordinate intraflagellar transport and ciliogenesis

Yujie Li, Qing Wei, Yuxia Zhang, Kun Ling, Jinghua Hu

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Intraflagellar transport (IFT) machinery mediates the bi-directional movement of cargos that are required for the assembly and maintenance of cilia. However, little is known about how IFT is regulated in vivo. In this study, we show that the small guanosine triphosphatase (GTPase) adenosine diphosphate ribosylation factor-like protein 13 (ARL-13) encoded by the Caenorhabditis elegans homologue of the human Joubert syndrome causal gene ARL13B, localizes exclusively to the doublet segment of the cilium. arl-13 mutants have shortened cilia with various ultrastructural deformities and a disrupted association between IFT subcomplexes A and B. Intriguingly, depletion of ARL-3, another ciliary small GTPase, partially suppresses ciliogenesis defects in arl-13 mutants by indirectly restoring binding between IFT subcomplexes A and B. Rescue of arl-13 mutants by ARL-3 depletion is mediated by an HDAC6 deacetylase-dependent pathway. Thus, we propose that two conserved small GTPases, ARL-13 and ARL-3, coordinate to regulate IFT and that perturbing this balance results in cilia deformation.

Original languageEnglish (US)
Pages (from-to)1039-1051
Number of pages13
JournalJournal of Cell Biology
Volume189
Issue number6
DOIs
StatePublished - Jun 14 2010

ASJC Scopus subject areas

  • Cell Biology

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