The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase

José Ayté, José F. Leis, Adriana Herrera, Eric Tang, Helena Yang, James A. DeCaprio

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

In Schizosaccharomyces pombe, MBF is a DNA-binding complex suspected to activate the transcription of genes necessary for entry into S phase. The MBF complex contains both p85(cdc10) and p72(res1/sct1). To obtain a better understanding of how the MBF complex regulates gene expression at the G1/S transition, we have performed a genetic analysis of p72(res1). We determined that p72(res1) can bind specifically to the cdc22 promoter, when analyzed by gel mobility shift assay, and that the N-terminal 157 amino acids of p72(res1) are sufficient for this specific binding. When overexpressed in vivo, a fragment of p72(res1) containing this DNA-binding domain could rescue a strain carrying a temperature-sensitive cdc10 allele at the restrictive temperature as well as a strain with a cdc10 null allele. We also determined that the C-terminal region of p72(res1) is necessary and sufficient for binding to p85(cdc10). Overexpression of the cdc10-binding domain of p72(res1) leads to a G1 arrest with a cdc phenotype and a decrease on MBF activity. Overexpression of full-length p72(res1) also leads to a growth arrest that can be rescued by overexpression of p85(cdc10). These results imply that the MBF activity in vivo is dependent on the interaction of p85(cdc10) with p72(res1).

Original languageEnglish (US)
Pages (from-to)2589-2599
Number of pages11
JournalMolecular and cellular biology
Volume15
Issue number5
DOIs
StatePublished - May 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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