The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase

J. Ayte, J. F. Leis, A. Herrera, E. Tang, H. Yang, J. A. Decaprio

Research output: Contribution to journalArticle

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Abstract

In Schizosaccharomyces pombe, MBF is a DNA-binding complex suspected to activate the transcription of genes necessary for entry into S phase. The MBF complex contains both p85(cdc10) and p72(res1/sct1). To obtain a better understanding of how the MBF complex regulates gene expression at the G1/S transition, we have performed a genetic analysis of p72(res1). We determined that p72(res1) can bind specifically to the cdc22 promoter, when analyzed by gel mobility shift assay, and that the N-terminal 157 amino acids of p72(res1) are sufficient for this specific binding. When overexpressed in vivo, a fragment of p72(res1) containing this DNA-binding domain could rescue a strain carrying a temperature-sensitive cdc10 allele at the restrictive temperature as well as a strain with a cdc10 null allele. We also determined that the C-terminal region of p72(res1) is necessary and sufficient for binding to p85(cdc10). Overexpression of the cdc10-binding domain of p72(res1) leads to a G1 arrest with a cdc phenotype and a decrease on MBF activity. Overexpression of full-length p72(res1) also leads to a growth arrest that can be rescued by overexpression of p85(cdc10). These results imply that the MBF activity in vivo is dependent on the interaction of p85(cdc10) with p72(res1).

Original languageEnglish (US)
Pages (from-to)2589-2599
Number of pages11
JournalMolecular and Cellular Biology
Volume15
Issue number5
StatePublished - 1995
Externally publishedYes

Fingerprint

Schizosaccharomyces
S Phase
Alleles
Temperature
DNA
Electrophoretic Mobility Shift Assay
Gels
Phenotype
Gene Expression
Amino Acids
Growth
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Ayte, J., Leis, J. F., Herrera, A., Tang, E., Yang, H., & Decaprio, J. A. (1995). The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Molecular and Cellular Biology, 15(5), 2589-2599.

The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. / Ayte, J.; Leis, J. F.; Herrera, A.; Tang, E.; Yang, H.; Decaprio, J. A.

In: Molecular and Cellular Biology, Vol. 15, No. 5, 1995, p. 2589-2599.

Research output: Contribution to journalArticle

Ayte, J, Leis, JF, Herrera, A, Tang, E, Yang, H & Decaprio, JA 1995, 'The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase', Molecular and Cellular Biology, vol. 15, no. 5, pp. 2589-2599.
Ayte J, Leis JF, Herrera A, Tang E, Yang H, Decaprio JA. The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Molecular and Cellular Biology. 1995;15(5):2589-2599.
Ayte, J. ; Leis, J. F. ; Herrera, A. ; Tang, E. ; Yang, H. ; Decaprio, J. A. / The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 5. pp. 2589-2599.
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