The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing

Rebecca J. Burgess, Hui Zhou, Junhong Han, Qing Li, Zhiguo Zhang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In budding yeast, transcriptional silencing, which is important to regulate gene expression and maintain genome integrity, requires silent information regulator (Sir) proteins. In addition, Rtt106, a histone chaperone involved in nucleosome assembly, functions in transcriptional silencing. However, how transcriptional silencing is regulated during mitotic cell division is not well understood. We show that cells lacking Dia2, a component of the SCFDia2 E3 ubiquitin ligase involved in DNA replication, display defects in silencing at the telomere and HMR locus and that the F-box and C-terminal regions of Dia2, two regions important for Dia2's ubiquitylation activity, are required for proper transcriptional silencing at these loci. In addition, we show that Sir proteins are mislocalized in dia2Δ mutant cells. Mutations in Dia2 and Rtt106 result in a synergistic loss of silencing at the HMR locus and significant elevation of Sir4 proteins at the HMR locus, suggesting that silencing defects in dia2Δ mutant cells are due, at least in part, to the altered levels of Sir4 at silent chromatin. Supporting this idea, we show that SCFDia2 ubiquitylates Sir4 in vitro and in vivo. Furthermore, Sir4 binding to silent chromatin is dynamically regulated during the cell cycle, and this regulation is lost in dia2Δ mutant cells. These results demonstrate that the SCFDia2 complex is involved in transcriptional silencing, ubiquitylates Sir4, and regulates transcriptional silencing during the cell cycle.

Original languageEnglish (US)
Article numbere1002846
JournalPLoS Genetics
Volume8
Issue number7
DOIs
StatePublished - Jul 2012

Fingerprint

ubiquitin-protein ligase
Ubiquitin-Protein Ligases
loci
protein
defect
mutants
Chromatin
chromatin
cell cycle
Cell Cycle
Histone Chaperones
cells
Proteins
Saccharomycetales
nucleosomes
gene expression
yeast
proteins
Nucleosomes
mutation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing. / Burgess, Rebecca J.; Zhou, Hui; Han, Junhong; Li, Qing; Zhang, Zhiguo.

In: PLoS Genetics, Vol. 8, No. 7, e1002846, 07.2012.

Research output: Contribution to journalArticle

Burgess, Rebecca J. ; Zhou, Hui ; Han, Junhong ; Li, Qing ; Zhang, Zhiguo. / The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing. In: PLoS Genetics. 2012 ; Vol. 8, No. 7.
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