The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells

Roberto Paredes, Gloria Arriagada, Fernando Cruzat, Juan Olate, Andre J van Wijnen, Jane Lian, Gary Stein, Janet Stein, Martin Montecino

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Bone-specific transcription of the osteocalcin (OC) gene is principally regulated by the Runx2 transcription factor and further stimulated in response to 1α,25-dihydroxy Vitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A-C). Mutation of sites A and B, which flank the 1α,25-dihydroxy Vitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxy Vitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between VDR and Runx2 factors. Additionally, the transcriptional co-activator p300 is recruited to the OC promoter by Runx2 where it up-regulates both basal and 1α,25-dihydroxy Vitamin D3-enhanced OC expression. Here, we present an overview of how in osteoblastic cells expressing OC, Runx2 modulates the 1α,25-dihydroxy Vitamin D3-dependent stimulation of the OC promoter by first recruiting transcriptional co-activators and then by further stabilizing the interaction of the VDR with the VDRE.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume89-90
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Osteocalcin
Gene expression
Rats
Transcription Factors
Up-Regulation
Gene Expression
Transcription
Genes
Cholecalciferol
dihydroxy-vitamin D3
Bone
Cells
Bone and Bones
Mutation

Keywords

  • Osteocalcin
  • Runx2
  • Transcription
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells. / Paredes, Roberto; Arriagada, Gloria; Cruzat, Fernando; Olate, Juan; van Wijnen, Andre J; Lian, Jane; Stein, Gary; Stein, Janet; Montecino, Martin.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 89-90, 05.2004, p. 269-271.

Research output: Contribution to journalArticle

Paredes, Roberto ; Arriagada, Gloria ; Cruzat, Fernando ; Olate, Juan ; van Wijnen, Andre J ; Lian, Jane ; Stein, Gary ; Stein, Janet ; Montecino, Martin. / The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells. In: Journal of Steroid Biochemistry and Molecular Biology. 2004 ; Vol. 89-90. pp. 269-271.
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