The roles of interleukin 2 and interferon-γ in human B cell activation, growth and differentiation

Diane F Jelinek, J. B. Splawski, P. E. Lipsky

Research output: Contribution to journalArticle

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Abstract

The roles of interleukin 2 (IL 2) and interferon-γ (IFN-γ) in human peripheral blood B cell activation, growth and differentiation were examined. Highly purified B cells stimulated with Cowan I Staphylococcus aureus (SA) proliferated minimally and generated no immunoglobulin-secreting cells (ISC) without the addition of T cell supernatants (T sup) produced by mitogen-activated T cells. Recombinant IL 2 (rIL 2) alone was able to promote maximum proliferation and generation of ISC in cultures of highly purified SA-stimulated B cells when present from the initiation of the incubation. IFN-γ, by contrast, could not support either response alone. When a two-step culture system was employed to determine the effect(s) of T cell influences during both initial activation and in propagating the response following activation, it was found that B cells activated by SA alone subsequently responded maximally to T sup but only minimally to IL 2 and not at all to IFN-γ. However, the presence of T sup, rIL 2, or rIFN-γ during initial activation with SA was found to facilitate greatly the subsequent capacity of the activated B cells to proliferate and differentiate in response to either T sup or IL 2. These data suggest two distinct pathways of human B cell responsiveness. Activities in T sup other than IL 2 or IFN-γ can support the growth and differentiation of B cells initially activated with SA alone, whereas rIL 2 is capable of promoting these responses maximally only when B cells have been initially activated by SA in the presence of T cell lymphokines, such as IL 2 or IFN-γ. The results emphasize the role of specific T cell factors in determining the outcome of humoral immune responses.

Original languageEnglish (US)
Pages (from-to)925-932
Number of pages8
JournalEuropean Journal of Immunology
Volume16
Issue number8
StatePublished - 1986
Externally publishedYes

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Interferons
Interleukin-2
B-Lymphocytes
T-Lymphocytes
Staphylococcus aureus
Growth
Antibody-Producing Cells
TCF Transcription Factors
Lymphokines
Humoral Immunity
Mitogens
Blood Cells
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology

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The roles of interleukin 2 and interferon-γ in human B cell activation, growth and differentiation. / Jelinek, Diane F; Splawski, J. B.; Lipsky, P. E.

In: European Journal of Immunology, Vol. 16, No. 8, 1986, p. 925-932.

Research output: Contribution to journalArticle

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