The roles of a Th2 cytokine and CC chemokine in children with stable asthma

Potential implication in eosinophil degranulation

Chang K. Kim, Hirohito Kita, Zak Callaway, Hyo B. Kim, Jungi Choi, Takao Fujisawa, Bo M. Shin, Young Y. Koh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Th2 cytokine IL-5 and CC chemokine eotaxin are thought to be key regulators of eosinophils in bronchial asthma. However, their involvement in children with stable asthma (SA) has not been determined. We investigated the roles of IL-5 and eotaxin in eosinophil degranulation in children with SA. Induced sputum was obtained from 30 SA, 21 allergic rhinitis (AR), and 22 non-atopic healthy control (HC) children. We measured sputum levels of IL-5, eotaxin, and eosinophil indices [percentage eosinophils, eosinophil-derived neurotoxin (EDN), and eosinophil-cationic protein (ECP)]. We also examined correlations of IL-5 and eotaxin with eosinophil indices. Sputum percentage eosinophils and EDN and ECP levels were significantly higher in the SA group than in the HC group, while only the sputum EDN and ECP levels were significantly higher in the AR group than in the HC group. Unexpectedly, sputum levels of IL-5 were not significantly different among the three groups; however, the levels of eotaxin were higher in the SA group when compared to the HC group. No significant correlations were found between IL-5 and percentage eosinophils, EDN, or ECP levels; in contrast, eotaxin levels correlated significantly with percentage eosinophils (Rs=0.638; p=0.0001), EDN (Rs=0.522; p=0.003), and ECP levels (Rs=0.630 and p=0.0002). The elevated levels and good correlations of eotaxin with sputum eosinophil indices, and no elevation or correlation of IL-5 with these indices, suggest that CC chemokine eotaxin may play a more important role in eosinophil degranulation in children with SA.

Original languageEnglish (US)
JournalPediatric Allergy and Immunology
Volume21
Issue number4 PART2
DOIs
StatePublished - Jun 2010

Fingerprint

CC Chemokines
Eosinophils
Asthma
Eosinophil-Derived Neurotoxin
Interleukin-5
Cytokines
Eosinophil Cationic Protein
Sputum
Control Groups

Keywords

  • Eosinophil degranulation
  • Eosinophil indices
  • Eotaxin
  • Interleukin-5
  • Stable asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pediatrics, Perinatology, and Child Health
  • Immunology
  • Medicine(all)

Cite this

The roles of a Th2 cytokine and CC chemokine in children with stable asthma : Potential implication in eosinophil degranulation. / Kim, Chang K.; Kita, Hirohito; Callaway, Zak; Kim, Hyo B.; Choi, Jungi; Fujisawa, Takao; Shin, Bo M.; Koh, Young Y.

In: Pediatric Allergy and Immunology, Vol. 21, No. 4 PART2, 06.2010.

Research output: Contribution to journalArticle

Kim, Chang K. ; Kita, Hirohito ; Callaway, Zak ; Kim, Hyo B. ; Choi, Jungi ; Fujisawa, Takao ; Shin, Bo M. ; Koh, Young Y. / The roles of a Th2 cytokine and CC chemokine in children with stable asthma : Potential implication in eosinophil degranulation. In: Pediatric Allergy and Immunology. 2010 ; Vol. 21, No. 4 PART2.
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abstract = "Th2 cytokine IL-5 and CC chemokine eotaxin are thought to be key regulators of eosinophils in bronchial asthma. However, their involvement in children with stable asthma (SA) has not been determined. We investigated the roles of IL-5 and eotaxin in eosinophil degranulation in children with SA. Induced sputum was obtained from 30 SA, 21 allergic rhinitis (AR), and 22 non-atopic healthy control (HC) children. We measured sputum levels of IL-5, eotaxin, and eosinophil indices [percentage eosinophils, eosinophil-derived neurotoxin (EDN), and eosinophil-cationic protein (ECP)]. We also examined correlations of IL-5 and eotaxin with eosinophil indices. Sputum percentage eosinophils and EDN and ECP levels were significantly higher in the SA group than in the HC group, while only the sputum EDN and ECP levels were significantly higher in the AR group than in the HC group. Unexpectedly, sputum levels of IL-5 were not significantly different among the three groups; however, the levels of eotaxin were higher in the SA group when compared to the HC group. No significant correlations were found between IL-5 and percentage eosinophils, EDN, or ECP levels; in contrast, eotaxin levels correlated significantly with percentage eosinophils (Rs=0.638; p=0.0001), EDN (Rs=0.522; p=0.003), and ECP levels (Rs=0.630 and p=0.0002). The elevated levels and good correlations of eotaxin with sputum eosinophil indices, and no elevation or correlation of IL-5 with these indices, suggest that CC chemokine eotaxin may play a more important role in eosinophil degranulation in children with SA.",
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AB - Th2 cytokine IL-5 and CC chemokine eotaxin are thought to be key regulators of eosinophils in bronchial asthma. However, their involvement in children with stable asthma (SA) has not been determined. We investigated the roles of IL-5 and eotaxin in eosinophil degranulation in children with SA. Induced sputum was obtained from 30 SA, 21 allergic rhinitis (AR), and 22 non-atopic healthy control (HC) children. We measured sputum levels of IL-5, eotaxin, and eosinophil indices [percentage eosinophils, eosinophil-derived neurotoxin (EDN), and eosinophil-cationic protein (ECP)]. We also examined correlations of IL-5 and eotaxin with eosinophil indices. Sputum percentage eosinophils and EDN and ECP levels were significantly higher in the SA group than in the HC group, while only the sputum EDN and ECP levels were significantly higher in the AR group than in the HC group. Unexpectedly, sputum levels of IL-5 were not significantly different among the three groups; however, the levels of eotaxin were higher in the SA group when compared to the HC group. No significant correlations were found between IL-5 and percentage eosinophils, EDN, or ECP levels; in contrast, eotaxin levels correlated significantly with percentage eosinophils (Rs=0.638; p=0.0001), EDN (Rs=0.522; p=0.003), and ECP levels (Rs=0.630 and p=0.0002). The elevated levels and good correlations of eotaxin with sputum eosinophil indices, and no elevation or correlation of IL-5 with these indices, suggest that CC chemokine eotaxin may play a more important role in eosinophil degranulation in children with SA.

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