The role of two polymorphic H-2 domains for the generation of alloreactive and H-2 restricted CTL clones

G. J. Haemmerling, C. Weyand, J. Goronzy

Research output: Contribution to journalArticlepeer-review

Abstract

With a set of mc anti-H-2 antibodies to major, spatially separate polymorphic domains, A and B could be identified on the H-2K(k) molecule. Target inhibition by mcAb demonstrates that these two domains harbor also the target sites for alloreactive CTL, and also the restriction sites for H-2 restricted CTL. The majority of both alloreactive and H-2 restricted CTL obtained in mass cultures appeared to be reactive against cluster B determinants. However, in limiting dilution systems several distinct subpopulations were found which differed in their receptor repertoire. Thus, the low frequency populations were predominantly specific for cluster B while the high frequent population contained mainly CTL clones which recognize cluster A. The appearance of these cluster-specific subpopulations seems to be regulated by yet unknown suppressive mechanisms.

Original languageEnglish (US)
Pages (from-to)90-95
Number of pages6
JournalBehring Institute Mitteilungen
VolumeNo. 70
StatePublished - 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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