The Role of Physical Stabilization in Whole Blood Preservation

Keith H K Wong, Rebecca D. Sandlin, Thomas R. Carey, Kathleen L. Miller, Aaron T. Shank, Rahmi Oklu, Shyamala Maheswaran, Daniel A. Haber, Daniel Irimia, Shannon L. Stott, Mehmet Toner

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The rapid degradation of blood ex vivo imposes logistical limitations on the utilization of blood-borne cells in medical diagnostics and scientific investigations. A fundamental but overlooked aspect in the storage of this fluid tissue is blood settling, which induces physical stress and compaction, aggregates blood cells, and causes collateral damage due to leukocyte activation. Here we show that the polymer Ficoll 70 kDa stabilized blood samples and prevented blood settling over the course of 72 hours, primarily by inhibiting depletion-mediated red blood cell aggregation. Physical stabilization decreased echinocyte formation, improved leukocyte viability, and inhibited the release of neutrophil elastase-a marker of neutrophil extracellular trap formation. In addition, Ficoll-stabilized blood was compatible with common leukocyte enrichment techniques including red blood cell lysis and immunomagnetic purification. This study showed for the first time that blood settling can be prevented using polymers and has implications in diagnostics.

Original languageEnglish (US)
Article number21023
JournalScientific Reports
Volume6
DOIs
StatePublished - Feb 15 2016

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Blood Preservation
Ficoll
Leukocytes
Blood Cells
Polymers
Erythrocytes
Cell Aggregation
Leukocyte Elastase

ASJC Scopus subject areas

  • General

Cite this

Wong, K. H. K., Sandlin, R. D., Carey, T. R., Miller, K. L., Shank, A. T., Oklu, R., ... Toner, M. (2016). The Role of Physical Stabilization in Whole Blood Preservation. Scientific Reports, 6, [21023]. https://doi.org/10.1038/srep21023

The Role of Physical Stabilization in Whole Blood Preservation. / Wong, Keith H K; Sandlin, Rebecca D.; Carey, Thomas R.; Miller, Kathleen L.; Shank, Aaron T.; Oklu, Rahmi; Maheswaran, Shyamala; Haber, Daniel A.; Irimia, Daniel; Stott, Shannon L.; Toner, Mehmet.

In: Scientific Reports, Vol. 6, 21023, 15.02.2016.

Research output: Contribution to journalArticle

Wong, KHK, Sandlin, RD, Carey, TR, Miller, KL, Shank, AT, Oklu, R, Maheswaran, S, Haber, DA, Irimia, D, Stott, SL & Toner, M 2016, 'The Role of Physical Stabilization in Whole Blood Preservation', Scientific Reports, vol. 6, 21023. https://doi.org/10.1038/srep21023
Wong KHK, Sandlin RD, Carey TR, Miller KL, Shank AT, Oklu R et al. The Role of Physical Stabilization in Whole Blood Preservation. Scientific Reports. 2016 Feb 15;6. 21023. https://doi.org/10.1038/srep21023
Wong, Keith H K ; Sandlin, Rebecca D. ; Carey, Thomas R. ; Miller, Kathleen L. ; Shank, Aaron T. ; Oklu, Rahmi ; Maheswaran, Shyamala ; Haber, Daniel A. ; Irimia, Daniel ; Stott, Shannon L. ; Toner, Mehmet. / The Role of Physical Stabilization in Whole Blood Preservation. In: Scientific Reports. 2016 ; Vol. 6.
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