The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma

Vishal S. Chandan, William C. Faquin, David C. Wilbur, Kamal K. Khurana

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

BACKGROUND. CD57 (Leu7), a marker for natural killer lymphocytes, and glucose transporter-1 (GLUT-1), a facultative cell surface glucose transport protein, are expressed in a wide spectrum of epithelial malignancies. The usefulness of CD57 and GLUT-1 immunostaining was evaluated as an adjunct to assist in the diagnosis of papillary carcinoma in fine-needle aspirations (FNAs) of the thyroid. METHODS. Immunohistochemical staining for CD57 and GLUT-1 was performed on paraffin-embedded cell blocks of 50 thyroid FNA cases with the following cytologic diagnoses: 1) papillary carcinoma (15 cases); 2) atypical cytology, cannot exclude papillary carcinoma (14 cases); and 3) benign thyroid (21 cases). RESULTS. Fourteen of 15 cases with an unequivocal diagnosis of papillary carcinoma were positive for CD57 and 1 case was negative for CD57. Tissue follow-up confirmed papillary carcinoma in all 15 cases. Of the 14 cases with a diagnosis of atypical cytology, cannot exclude papillary carcinoma, 6 cases were positive for CD57 and subsequent excision confirmed papillary carcinoma in all 6 cases. The remaining 8 cases negative for CD57 included surgically confirmed goiter (5 cases), adenoma (2 cases), and papillary carcinoma (1 case). The follicular cells in all 21 cases with a cytologic diagnosis of benign thyroid were negative for CD57. Histologic follow-up of these cases confirmed the benign cytologic diagnoses. GLUT-1 was negative in all cases. The sensitivity and specificity of CD57 was 91% and 100%, respectively. CONCLUSIONS. CD57 immunostaining is a useful adjunct for the diagnosis of papillary thyroid carcinoma in cell block material. Although negative staining for CD57 does not completely exclude papillary carcinoma, positive staining aids in the accurate diagnosis of malignancy in cytomorphologically equivocal cases. GLUT-1 did not prove to be a useful marker in distinguishing papillary carcinomas.

Original languageEnglish (US)
Pages (from-to)331-336
Number of pages6
JournalCancer
Volume108
Issue number5
DOIs
StatePublished - Oct 25 2006
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Papillary Carcinoma
Fine Needle Biopsy
Thyroid Gland
Cell Biology
Papillary Thyroid cancer
Staining and Labeling
Negative Staining
Goiter
Adenoma
Paraffin
Neoplasms
Lymphocytes
Sensitivity and Specificity

Keywords

  • CD57
  • Cytology
  • Fine-needle aspiration
  • GLUT-1
  • Immunohistochemistry
  • Papillary carcinoma
  • Thyroid

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma. / Chandan, Vishal S.; Faquin, William C.; Wilbur, David C.; Khurana, Kamal K.

In: Cancer, Vol. 108, No. 5, 25.10.2006, p. 331-336.

Research output: Contribution to journalArticle

Chandan, Vishal S. ; Faquin, William C. ; Wilbur, David C. ; Khurana, Kamal K. / The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma. In: Cancer. 2006 ; Vol. 108, No. 5. pp. 331-336.
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title = "The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma",
abstract = "BACKGROUND. CD57 (Leu7), a marker for natural killer lymphocytes, and glucose transporter-1 (GLUT-1), a facultative cell surface glucose transport protein, are expressed in a wide spectrum of epithelial malignancies. The usefulness of CD57 and GLUT-1 immunostaining was evaluated as an adjunct to assist in the diagnosis of papillary carcinoma in fine-needle aspirations (FNAs) of the thyroid. METHODS. Immunohistochemical staining for CD57 and GLUT-1 was performed on paraffin-embedded cell blocks of 50 thyroid FNA cases with the following cytologic diagnoses: 1) papillary carcinoma (15 cases); 2) atypical cytology, cannot exclude papillary carcinoma (14 cases); and 3) benign thyroid (21 cases). RESULTS. Fourteen of 15 cases with an unequivocal diagnosis of papillary carcinoma were positive for CD57 and 1 case was negative for CD57. Tissue follow-up confirmed papillary carcinoma in all 15 cases. Of the 14 cases with a diagnosis of atypical cytology, cannot exclude papillary carcinoma, 6 cases were positive for CD57 and subsequent excision confirmed papillary carcinoma in all 6 cases. The remaining 8 cases negative for CD57 included surgically confirmed goiter (5 cases), adenoma (2 cases), and papillary carcinoma (1 case). The follicular cells in all 21 cases with a cytologic diagnosis of benign thyroid were negative for CD57. Histologic follow-up of these cases confirmed the benign cytologic diagnoses. GLUT-1 was negative in all cases. The sensitivity and specificity of CD57 was 91{\%} and 100{\%}, respectively. CONCLUSIONS. CD57 immunostaining is a useful adjunct for the diagnosis of papillary thyroid carcinoma in cell block material. Although negative staining for CD57 does not completely exclude papillary carcinoma, positive staining aids in the accurate diagnosis of malignancy in cytomorphologically equivocal cases. GLUT-1 did not prove to be a useful marker in distinguishing papillary carcinomas.",
keywords = "CD57, Cytology, Fine-needle aspiration, GLUT-1, Immunohistochemistry, Papillary carcinoma, Thyroid",
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T1 - The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma

AU - Chandan, Vishal S.

AU - Faquin, William C.

AU - Wilbur, David C.

AU - Khurana, Kamal K.

PY - 2006/10/25

Y1 - 2006/10/25

N2 - BACKGROUND. CD57 (Leu7), a marker for natural killer lymphocytes, and glucose transporter-1 (GLUT-1), a facultative cell surface glucose transport protein, are expressed in a wide spectrum of epithelial malignancies. The usefulness of CD57 and GLUT-1 immunostaining was evaluated as an adjunct to assist in the diagnosis of papillary carcinoma in fine-needle aspirations (FNAs) of the thyroid. METHODS. Immunohistochemical staining for CD57 and GLUT-1 was performed on paraffin-embedded cell blocks of 50 thyroid FNA cases with the following cytologic diagnoses: 1) papillary carcinoma (15 cases); 2) atypical cytology, cannot exclude papillary carcinoma (14 cases); and 3) benign thyroid (21 cases). RESULTS. Fourteen of 15 cases with an unequivocal diagnosis of papillary carcinoma were positive for CD57 and 1 case was negative for CD57. Tissue follow-up confirmed papillary carcinoma in all 15 cases. Of the 14 cases with a diagnosis of atypical cytology, cannot exclude papillary carcinoma, 6 cases were positive for CD57 and subsequent excision confirmed papillary carcinoma in all 6 cases. The remaining 8 cases negative for CD57 included surgically confirmed goiter (5 cases), adenoma (2 cases), and papillary carcinoma (1 case). The follicular cells in all 21 cases with a cytologic diagnosis of benign thyroid were negative for CD57. Histologic follow-up of these cases confirmed the benign cytologic diagnoses. GLUT-1 was negative in all cases. The sensitivity and specificity of CD57 was 91% and 100%, respectively. CONCLUSIONS. CD57 immunostaining is a useful adjunct for the diagnosis of papillary thyroid carcinoma in cell block material. Although negative staining for CD57 does not completely exclude papillary carcinoma, positive staining aids in the accurate diagnosis of malignancy in cytomorphologically equivocal cases. GLUT-1 did not prove to be a useful marker in distinguishing papillary carcinomas.

AB - BACKGROUND. CD57 (Leu7), a marker for natural killer lymphocytes, and glucose transporter-1 (GLUT-1), a facultative cell surface glucose transport protein, are expressed in a wide spectrum of epithelial malignancies. The usefulness of CD57 and GLUT-1 immunostaining was evaluated as an adjunct to assist in the diagnosis of papillary carcinoma in fine-needle aspirations (FNAs) of the thyroid. METHODS. Immunohistochemical staining for CD57 and GLUT-1 was performed on paraffin-embedded cell blocks of 50 thyroid FNA cases with the following cytologic diagnoses: 1) papillary carcinoma (15 cases); 2) atypical cytology, cannot exclude papillary carcinoma (14 cases); and 3) benign thyroid (21 cases). RESULTS. Fourteen of 15 cases with an unequivocal diagnosis of papillary carcinoma were positive for CD57 and 1 case was negative for CD57. Tissue follow-up confirmed papillary carcinoma in all 15 cases. Of the 14 cases with a diagnosis of atypical cytology, cannot exclude papillary carcinoma, 6 cases were positive for CD57 and subsequent excision confirmed papillary carcinoma in all 6 cases. The remaining 8 cases negative for CD57 included surgically confirmed goiter (5 cases), adenoma (2 cases), and papillary carcinoma (1 case). The follicular cells in all 21 cases with a cytologic diagnosis of benign thyroid were negative for CD57. Histologic follow-up of these cases confirmed the benign cytologic diagnoses. GLUT-1 was negative in all cases. The sensitivity and specificity of CD57 was 91% and 100%, respectively. CONCLUSIONS. CD57 immunostaining is a useful adjunct for the diagnosis of papillary thyroid carcinoma in cell block material. Although negative staining for CD57 does not completely exclude papillary carcinoma, positive staining aids in the accurate diagnosis of malignancy in cytomorphologically equivocal cases. GLUT-1 did not prove to be a useful marker in distinguishing papillary carcinomas.

KW - CD57

KW - Cytology

KW - Fine-needle aspiration

KW - GLUT-1

KW - Immunohistochemistry

KW - Papillary carcinoma

KW - Thyroid

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