The role of dynamin 3 in the testis

K. S. Vaid, J. A. Guttman, N. Babyak, W. Deng, Mark A Mc Niven, N. Mochizuki, B. B. Finlay, A. W. Vogl

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We report here that dynamin 3 in the testis is associated with structures termed tubulobulbar complexes that internalize intact intercellular junctions during sperm release and turnover of the blood-testis barrier. The protein lies adjacent to an actin-Arp2/3 network that cuffs the double plasma membrane tubular invagination at the core of each complex. To explore the possible relationship between dynamin 3 and nectin-based adhesion junctions, we transiently transfected DsRed-tagged dynamin 3 into MDCK eel Is stably transfected with eGFP-tagged nectin 2, one of the adhesion molecules known to be expressed in Sertoli cells at adhesion junctions. Cells transfected with the dynamin 3 construct had less uniformly distributed nectin 2 at intercellular contacts when compared to control cells expressing only nectin 2 or transfected with the DsRed plasmid alone. Significantly, tubular extensions positive for nectin 2 were visible projecting into the cells from regions of intercellular contact. Our findings are consistent with the conclusion that dynamin 3 is involved with tubulobulbar morphogenesis. Dynamin 3 also occurs in concentrated deposits around the capitulum and striated columns in the connecting piece of sperm tails suggesting that the protein in these cells may function to stabilize the base of the tail or serve as a reservoir for use during or after fertilization.

Original languageEnglish (US)
Pages (from-to)644-654
Number of pages11
JournalJournal of Cellular Physiology
Volume210
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Dynamin III
Testis
Adhesion
Blood-Testis Barrier
Sperm Tail
Eels
Intercellular Junctions
Sertoli Cells
Cell membranes
Morphogenesis
Fertilization
Cell Adhesion
Spermatozoa
Tail
Actins
Proteins
Plasmids
Blood
Deposits
Cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Vaid, K. S., Guttman, J. A., Babyak, N., Deng, W., Mc Niven, M. A., Mochizuki, N., ... Vogl, A. W. (2007). The role of dynamin 3 in the testis. Journal of Cellular Physiology, 210(3), 644-654. https://doi.org/10.1002/jcp.20855

The role of dynamin 3 in the testis. / Vaid, K. S.; Guttman, J. A.; Babyak, N.; Deng, W.; Mc Niven, Mark A; Mochizuki, N.; Finlay, B. B.; Vogl, A. W.

In: Journal of Cellular Physiology, Vol. 210, No. 3, 03.2007, p. 644-654.

Research output: Contribution to journalArticle

Vaid, KS, Guttman, JA, Babyak, N, Deng, W, Mc Niven, MA, Mochizuki, N, Finlay, BB & Vogl, AW 2007, 'The role of dynamin 3 in the testis', Journal of Cellular Physiology, vol. 210, no. 3, pp. 644-654. https://doi.org/10.1002/jcp.20855
Vaid KS, Guttman JA, Babyak N, Deng W, Mc Niven MA, Mochizuki N et al. The role of dynamin 3 in the testis. Journal of Cellular Physiology. 2007 Mar;210(3):644-654. https://doi.org/10.1002/jcp.20855
Vaid, K. S. ; Guttman, J. A. ; Babyak, N. ; Deng, W. ; Mc Niven, Mark A ; Mochizuki, N. ; Finlay, B. B. ; Vogl, A. W. / The role of dynamin 3 in the testis. In: Journal of Cellular Physiology. 2007 ; Vol. 210, No. 3. pp. 644-654.
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