The role of 5-HTT LPR and GNβ3 825C>T polymorphisms and gene-environment interactions in irritable bowel syndrome (IBS)

Yuri Ann Saito Loftus, Joseph J. Larson, Elizabeth J. Atkinson, Euijung Ryu, Ann E. Almazar, Gloria M Petersen, Nicholas J. Talley

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background Smaller studies have evaluated SLC6A4 5-HTTLPR and GNβ3 825C[T polymorphisms in IBS, and interactions between 5-HTT LPR with life events have been reported in the psychiatric literature, but gene-environment studies in IBS are lacking. Aims The purpose of this study was to assess the association of two polymorphisms with IBS and age of onset, and whether there are gene-environment interactions with IBS. Methods Outpatients with IBS and controls completed a validated questionnaire and provided blood for DNA. Comparisons of genotype/allele frequencies between cases and controls were performed with logistic regression. Linear regression was used to evaluate the association between the variants and age of onset. Environmental variables tested included abuse, parental alcohol abuse, parental psychiatric disorders, and gastrointestinal infections. Results Genotyping was performed in 385 cases and 262 controls with median age of 50 years (range, 18.0-70.0) and 498 (77%) females. The IBS subtype distribution among cases was: 102 (26%) D-IBS, 40 (10%) C-IBS, 125 (32%) M-IBS, 118 (31%) other. No association was observed between IBS or age of onset and both variants. Significant interactions were observed between GI infection and the GNb3 825T allele. For those reporting gastrointestinal infection, the OR for IBS was 3.9 (95% CI 1.2-12.7) whereas the OR was 0.86 (95% CI 0.65-1.13) for those without prior infection. Conclusions There was a significant interaction between the GNb3 polymorphism and infection in the development of IBS, suggesting that its etiology is the result of a combination of specific genetic and environmental risk factors.

Original languageEnglish (US)
Pages (from-to)2650-2657
Number of pages8
JournalDigestive Diseases and Sciences
Volume57
Issue number10
DOIs
StatePublished - Oct 2012

Fingerprint

Gene-Environment Interaction
Irritable Bowel Syndrome
Age of Onset
Infection
Psychiatry
Gene Frequency
Alcoholism
Linear Models
Outpatients
Logistic Models

Keywords

  • Genes
  • Genetics
  • Infection
  • Irritable bowel syndrome

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

The role of 5-HTT LPR and GNβ3 825C>T polymorphisms and gene-environment interactions in irritable bowel syndrome (IBS). / Saito Loftus, Yuri Ann; Larson, Joseph J.; Atkinson, Elizabeth J.; Ryu, Euijung; Almazar, Ann E.; Petersen, Gloria M; Talley, Nicholas J.

In: Digestive Diseases and Sciences, Vol. 57, No. 10, 10.2012, p. 2650-2657.

Research output: Contribution to journalArticle

@article{7d81ac27c9444afeba7793c4adc6203a,
title = "The role of 5-HTT LPR and GNβ3 825C>T polymorphisms and gene-environment interactions in irritable bowel syndrome (IBS)",
abstract = "Background Smaller studies have evaluated SLC6A4 5-HTTLPR and GNβ3 825C[T polymorphisms in IBS, and interactions between 5-HTT LPR with life events have been reported in the psychiatric literature, but gene-environment studies in IBS are lacking. Aims The purpose of this study was to assess the association of two polymorphisms with IBS and age of onset, and whether there are gene-environment interactions with IBS. Methods Outpatients with IBS and controls completed a validated questionnaire and provided blood for DNA. Comparisons of genotype/allele frequencies between cases and controls were performed with logistic regression. Linear regression was used to evaluate the association between the variants and age of onset. Environmental variables tested included abuse, parental alcohol abuse, parental psychiatric disorders, and gastrointestinal infections. Results Genotyping was performed in 385 cases and 262 controls with median age of 50 years (range, 18.0-70.0) and 498 (77{\%}) females. The IBS subtype distribution among cases was: 102 (26{\%}) D-IBS, 40 (10{\%}) C-IBS, 125 (32{\%}) M-IBS, 118 (31{\%}) other. No association was observed between IBS or age of onset and both variants. Significant interactions were observed between GI infection and the GNb3 825T allele. For those reporting gastrointestinal infection, the OR for IBS was 3.9 (95{\%} CI 1.2-12.7) whereas the OR was 0.86 (95{\%} CI 0.65-1.13) for those without prior infection. Conclusions There was a significant interaction between the GNb3 polymorphism and infection in the development of IBS, suggesting that its etiology is the result of a combination of specific genetic and environmental risk factors.",
keywords = "Genes, Genetics, Infection, Irritable bowel syndrome",
author = "{Saito Loftus}, {Yuri Ann} and Larson, {Joseph J.} and Atkinson, {Elizabeth J.} and Euijung Ryu and Almazar, {Ann E.} and Petersen, {Gloria M} and Talley, {Nicholas J.}",
year = "2012",
month = "10",
doi = "10.1007/s10620-012-2319-9",
language = "English (US)",
volume = "57",
pages = "2650--2657",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "10",

}

TY - JOUR

T1 - The role of 5-HTT LPR and GNβ3 825C>T polymorphisms and gene-environment interactions in irritable bowel syndrome (IBS)

AU - Saito Loftus, Yuri Ann

AU - Larson, Joseph J.

AU - Atkinson, Elizabeth J.

AU - Ryu, Euijung

AU - Almazar, Ann E.

AU - Petersen, Gloria M

AU - Talley, Nicholas J.

PY - 2012/10

Y1 - 2012/10

N2 - Background Smaller studies have evaluated SLC6A4 5-HTTLPR and GNβ3 825C[T polymorphisms in IBS, and interactions between 5-HTT LPR with life events have been reported in the psychiatric literature, but gene-environment studies in IBS are lacking. Aims The purpose of this study was to assess the association of two polymorphisms with IBS and age of onset, and whether there are gene-environment interactions with IBS. Methods Outpatients with IBS and controls completed a validated questionnaire and provided blood for DNA. Comparisons of genotype/allele frequencies between cases and controls were performed with logistic regression. Linear regression was used to evaluate the association between the variants and age of onset. Environmental variables tested included abuse, parental alcohol abuse, parental psychiatric disorders, and gastrointestinal infections. Results Genotyping was performed in 385 cases and 262 controls with median age of 50 years (range, 18.0-70.0) and 498 (77%) females. The IBS subtype distribution among cases was: 102 (26%) D-IBS, 40 (10%) C-IBS, 125 (32%) M-IBS, 118 (31%) other. No association was observed between IBS or age of onset and both variants. Significant interactions were observed between GI infection and the GNb3 825T allele. For those reporting gastrointestinal infection, the OR for IBS was 3.9 (95% CI 1.2-12.7) whereas the OR was 0.86 (95% CI 0.65-1.13) for those without prior infection. Conclusions There was a significant interaction between the GNb3 polymorphism and infection in the development of IBS, suggesting that its etiology is the result of a combination of specific genetic and environmental risk factors.

AB - Background Smaller studies have evaluated SLC6A4 5-HTTLPR and GNβ3 825C[T polymorphisms in IBS, and interactions between 5-HTT LPR with life events have been reported in the psychiatric literature, but gene-environment studies in IBS are lacking. Aims The purpose of this study was to assess the association of two polymorphisms with IBS and age of onset, and whether there are gene-environment interactions with IBS. Methods Outpatients with IBS and controls completed a validated questionnaire and provided blood for DNA. Comparisons of genotype/allele frequencies between cases and controls were performed with logistic regression. Linear regression was used to evaluate the association between the variants and age of onset. Environmental variables tested included abuse, parental alcohol abuse, parental psychiatric disorders, and gastrointestinal infections. Results Genotyping was performed in 385 cases and 262 controls with median age of 50 years (range, 18.0-70.0) and 498 (77%) females. The IBS subtype distribution among cases was: 102 (26%) D-IBS, 40 (10%) C-IBS, 125 (32%) M-IBS, 118 (31%) other. No association was observed between IBS or age of onset and both variants. Significant interactions were observed between GI infection and the GNb3 825T allele. For those reporting gastrointestinal infection, the OR for IBS was 3.9 (95% CI 1.2-12.7) whereas the OR was 0.86 (95% CI 0.65-1.13) for those without prior infection. Conclusions There was a significant interaction between the GNb3 polymorphism and infection in the development of IBS, suggesting that its etiology is the result of a combination of specific genetic and environmental risk factors.

KW - Genes

KW - Genetics

KW - Infection

KW - Irritable bowel syndrome

UR - http://www.scopus.com/inward/record.url?scp=84867864859&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867864859&partnerID=8YFLogxK

U2 - 10.1007/s10620-012-2319-9

DO - 10.1007/s10620-012-2319-9

M3 - Article

C2 - 22855291

AN - SCOPUS:84867864859

VL - 57

SP - 2650

EP - 2657

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 10

ER -