The regulation of apoptosis by the downstream regulatory element antagonist modulator/potassium channel interacting protein 3 (DREAM/KChIP3) through interactions with hexokinase I

Theodore A. Craig, Pradeep L. Ramachandran, H. Robert Bergen, Jewel L. Podratz, Anthony J. Windebank, Rajiv Kumar

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The EF-hand protein, DREAM/KChIP3 (henceforth referred to as DREAM), regulates apoptosis by incompletely understood mechanisms. We demonstrate that in the presence of Ca2+, DREAM interacts with hexokinase I, a protein known to bind mitochondria and regulate apoptosis. A mutant DREAM protein construct incapable of binding Ca2+ does not associate with hexokinase I. The amino-terminal portion of DREAM is required for binding to hexokinase I, as a DREAM construct lacking the first 94 amino terminal residues fails to bind hexokinase I. Expression of DREAM in neuroblastoma cells enhances cisplatin mediated caspase-3 activity. Simultaneous expression of hexokinase I in such cells reduces DREAM-stimulated apoptosis. DREAM overexpression in neuroblastoma cells reduces hexokinase I localization on isolated mitochondria. The interaction of DREAM with hexokinase I may be important in the regulation of neuronal apoptosis.

Original languageEnglish (US)
Pages (from-to)508-512
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume433
Issue number4
DOIs
StatePublished - Apr 19 2013

Keywords

  • Apoptosis
  • Calcium
  • Calsenilin
  • DREAM
  • EF-hand proteins
  • Hexokinase I
  • KChIP3
  • Neuronal calcium sensor proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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