The reaction between nitric oxide and α-tocopheuol: A reappraisal

Neil Hogg; Ravinder Jit Singh; Steven P.A. Goss; B. Kalyanaraman

doi:10.1006/bbrc.1996.1086

The reaction between nitric oxide and α-tocopheuol: A reappraisal

Neil Hogg, Ravinder Jit Singh, Steven P.A. Goss, B. Kalyanaraman

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.

Original language	English (US)
Pages (from-to)	696-702
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	224
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1996.1086
State	Published - Jul 25 1996

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The reaction between nitric oxide and α-tocopheuol: A reappraisal",

abstract = "Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.",

author = "Neil Hogg and Singh, {Ravinder Jit} and Goss, {Steven P.A.} and B. Kalyanaraman",

note = "Funding Information: This research was supported by HL47250 from the National Heart, Lung and Blood Institute and RR01008.",

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AU - Goss, Steven P.A.

AU - Kalyanaraman, B.

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N2 - Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.

AB - Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.

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The reaction between nitric oxide and α-tocopheuol: A reappraisal

Abstract

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