Recently Gorbunov et al. reported that nitric oxide (·NO) can directly oxidize α-tocopherol to α-tocopheroxyl radical. We have reinvestigated this reaction and report that a direct reaction between ·NO and a-tocopherol does not occur. However, the reaction between ·NO and oxygen generates an oxidant which oxidizes α-tocopherol to α-tocopheryl quinone. Exposure of α-tocopherol to a low flux of ·NO generated from spermine NONOnte (100 μM) results in no consumption of α-tocopherol under either aerobic or anaerobic conditions. A higher flux of ·NO, generated from 1 mM spermine NONOate, oxidizes α-tocopherol only under aerobic conditions. Artifactual oxidation of α-tocopherol can be observed when using commercial ·NO that is contaminated with higher oxides of nitrogen, such as dinitrogen trioxide and dinitrogen tetraoxide.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 25 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology