The putative Wnt receptor Xenopus frizzled-7 functions upstream of β-catenin in vertebrate dorsoventral mesoderm patterning

Saulius Sumanas, Peter Strege, Janet Heasman, Stephen C Ekker

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

We have isolated one member of the frizzled family of wnt receptors from Xenopus (Xfz7) to study the role of cell-cell communication in the establishment of the vertebrate axis. We demonstrate that this maternally encoded protein specifically synergizes with wnt proteins in ectopic axis induction. Embryos derived from oocytes depleted of maternal Xfz7 RNA by antisense oligonucleotide injection are deficient in dorsoanterior structures. Xfz7-depleted embryos are deficient in dorsal but not ventral mesoderm due to the reduced expression of the wnt target genes siamois, Xnr3 and goosecoid. These signaling defects can be restored by the addition of β-catenin but not Xwnt8b. Xfz7 thus functions upstream of the known GSK-3/axin/β-catenin intracellular signaling complex in vertebrate dorsoventral mesoderm specification.

Original languageEnglish (US)
Pages (from-to)1981-1990
Number of pages10
JournalDevelopment
Volume127
Issue number9
StatePublished - May 2000
Externally publishedYes

Fingerprint

Wnt Receptors
Catenins
Mesoderm
Xenopus
Vertebrates
Embryonic Structures
Wnt Proteins
Glycogen Synthase Kinase 3
Antisense Oligonucleotides
Cell Communication
Oocytes
Mothers
RNA
Injections
Genes
Proteins

Keywords

  • β-catenin
  • Axis specification
  • Dorsal mesoderm
  • frizzled
  • wnt
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The putative Wnt receptor Xenopus frizzled-7 functions upstream of β-catenin in vertebrate dorsoventral mesoderm patterning. / Sumanas, Saulius; Strege, Peter; Heasman, Janet; Ekker, Stephen C.

In: Development, Vol. 127, No. 9, 05.2000, p. 1981-1990.

Research output: Contribution to journalArticle

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