The osteocalcin gene promoter provides a molecular blueprint for regulatory mechanisms controlling bone tissue formation: Role of transcription factors involved in development

Jane B. Lian, Gary S. Stein, Janet L. Stein, André Van Wijnen, Laura McCabe, Chaitali Banerjee, Heidi Hoffmann

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Characterization of regulatory sequences and their cognate binding factors in the bone-specific osteocalcin (OC) gene promoter has provided insight into mechanisms that control expression of the gene under diverse biological conditions. We present evidence for AP-1 motifs and two multipartite conserved regulatory sequences, the OC Box I (nt -99 to -76) and a site designated OC Box II (nt -136 to -130) in contributing to developmental and tissue-specific expression of osteocalcin. OC Box I is characterized by a homeodomain binding site and OC Box II is a recognition sequence for AML-1 (also called PEBP2α), a runt homology-related DNA binding protein. Functional activity of the elements was established in osseous and non-osseous cell lines and is in part related to the binding of osteoblast-specific complexes which enhance OC transcription. The contribution of several elements and binding of multiple classes of transcription factors to independent elements in both these domains serve to illustrate the complexity of control required for tissue-specific OC expression.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalConnective Tissue Research
Volume35
Issue number1-4
DOIs
StatePublished - 1996

Keywords

  • AML-I/PEBP2α
  • Bone specific gene expression
  • Fos/Jun proteins
  • Homeodomain proteins
  • MSX-2

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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