TY - JOUR
T1 - The origin of hepatitis C virus reinfecting transplanted livers
T2 - Serum-derived versus peripheral blood mononuclear cell-derived virus
AU - Laskus, Tomasz
AU - Radkowski, Marek
AU - Wilkinson, Jeffrey
AU - Vargas, Hugo
AU - Rakela, Jorge
PY - 2002/2/15
Y1 - 2002/2/15
N2 - When hepatitis C virus (HCV) infection recurs after liver transplantation, it is unclear whether the liver graft is colonized by virions present in the circulation or by those associated with peripheral blood mononuclear cells (PBMC). In 6 HCV-infected transplant recipients, HCV sequences were analyzed by the single-strand conformational polymorphism (SSCP) assay and direct sequencing in pretransplant-paired PBMC and serum samples and in posttransplant follow-up serum samples. In 2 patients, SSCP patterns for pretransplant PBMC-serum pairs were identical, while in 4 patients they were different. In 3 patients from the latter group, the posttransplant viral sequences resembled those found in pretransplant serum samples, whereas in the other patient from that group, viral sequences after transplantation were transiently identical to those found in pretransplant PBMC. In HCV-positive liver transplant recipients, the liver graft is colonized primarily by liver-derived virus remaining in the circulation. However, virus variants of likely extrahepatic origin can be detected in serum early after transplantation.
AB - When hepatitis C virus (HCV) infection recurs after liver transplantation, it is unclear whether the liver graft is colonized by virions present in the circulation or by those associated with peripheral blood mononuclear cells (PBMC). In 6 HCV-infected transplant recipients, HCV sequences were analyzed by the single-strand conformational polymorphism (SSCP) assay and direct sequencing in pretransplant-paired PBMC and serum samples and in posttransplant follow-up serum samples. In 2 patients, SSCP patterns for pretransplant PBMC-serum pairs were identical, while in 4 patients they were different. In 3 patients from the latter group, the posttransplant viral sequences resembled those found in pretransplant serum samples, whereas in the other patient from that group, viral sequences after transplantation were transiently identical to those found in pretransplant PBMC. In HCV-positive liver transplant recipients, the liver graft is colonized primarily by liver-derived virus remaining in the circulation. However, virus variants of likely extrahepatic origin can be detected in serum early after transplantation.
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U2 - 10.1086/338635
DO - 10.1086/338635
M3 - Article
C2 - 11865392
AN - SCOPUS:0037083253
SN - 0022-1899
VL - 185
SP - 417
EP - 421
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -