The nucleosome map of the mammalian liver

Zhaoyu Li, Jonathan Schug, Geetu Tuteja, Peter White, Klaus H. Kaestner

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Abstract

Binding to nucleosomal DNA is critical for 'pioneer' transcription factors such as the winged-helix transcription factors Foxa1 and Foxa2 to regulate chromatin structure and gene activation. Here we report the genome-wide map of nucleosome positions in the mouse liver, with emphasis on transcriptional start sites, CpG islands, Foxa2 binding sites and their correlation with gene expression. Despite the heterogeneity of liver tissue, we could clearly discern the nucleosome pattern of the predominant liver cell, the hepatocyte. By analyzing nucleosome occupancy and the distributions of heterochromatin protein 1 (Hp1), CBP (also known as Crebbp) and p300 (Ep300) in Foxa1- and Foxa2-deficient livers, we find that the maintenance of nucleosome position and chromatin structure surrounding Foxa2 binding sites is independent of Foxa1 and Foxa2.

Original languageEnglish (US)
Pages (from-to)742-746
Number of pages5
JournalNature Structural and Molecular Biology
Volume18
Issue number6
DOIs
StatePublished - Jun 1 2011

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ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Li, Z., Schug, J., Tuteja, G., White, P., & Kaestner, K. H. (2011). The nucleosome map of the mammalian liver. Nature Structural and Molecular Biology, 18(6), 742-746. https://doi.org/10.1038/nsmb.2060