The nuclear matrix protein NMP-1 is the transcription factor YY1

Bo Guo, Paul R. Odgren, Andre J van Wijnen, Thomas J. Last, Jeffrey Nickerson, Sheldon Penman, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm thai NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)10526-10530
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number23
DOIs
StatePublished - Nov 7 1995
Externally publishedYes

Fingerprint

YY1 Transcription Factor
Nuclear Matrix
Western Blotting
Gene Expression Regulation
Electrophoretic Mobility Shift Assay
Osteosarcoma
Histones
Genes
Fluorescent Antibody Technique
Proteins
Gels
Antibodies
DNA

Keywords

  • gene expression
  • histone H4
  • nuclear structure
  • nucleolus
  • osteoblast

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Guo, B., Odgren, P. R., van Wijnen, A. J., Last, T. J., Nickerson, J., Penman, S., ... Stein, G. S. (1995). The nuclear matrix protein NMP-1 is the transcription factor YY1. Proceedings of the National Academy of Sciences of the United States of America, 92(23), 10526-10530. https://doi.org/10.1073/pnas.92.23.10526

The nuclear matrix protein NMP-1 is the transcription factor YY1. / Guo, Bo; Odgren, Paul R.; van Wijnen, Andre J; Last, Thomas J.; Nickerson, Jeffrey; Penman, Sheldon; Lian, Jane B.; Stein, Janet L.; Stein, Gary S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 23, 07.11.1995, p. 10526-10530.

Research output: Contribution to journalArticle

Guo, B, Odgren, PR, van Wijnen, AJ, Last, TJ, Nickerson, J, Penman, S, Lian, JB, Stein, JL & Stein, GS 1995, 'The nuclear matrix protein NMP-1 is the transcription factor YY1', Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 23, pp. 10526-10530. https://doi.org/10.1073/pnas.92.23.10526
Guo, Bo ; Odgren, Paul R. ; van Wijnen, Andre J ; Last, Thomas J. ; Nickerson, Jeffrey ; Penman, Sheldon ; Lian, Jane B. ; Stein, Janet L. ; Stein, Gary S. / The nuclear matrix protein NMP-1 is the transcription factor YY1. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 23. pp. 10526-10530.
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