The nuclear matrix protein NMP-1 is the transcription factor YY1

Bo Guo, Paul R. Odgren, Andre J van Wijnen, Thomas J. Last, Jeffrey Nickerson, Sheldon Penman, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

159 Scopus citations


NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm thai NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)10526-10530
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number23
StatePublished - Nov 7 1995
Externally publishedYes



  • gene expression
  • histone H4
  • nuclear structure
  • nucleolus
  • osteoblast

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Guo, B., Odgren, P. R., van Wijnen, A. J., Last, T. J., Nickerson, J., Penman, S., Lian, J. B., Stein, J. L., & Stein, G. S. (1995). The nuclear matrix protein NMP-1 is the transcription factor YY1. Proceedings of the National Academy of Sciences of the United States of America, 92(23), 10526-10530.