The myosin cardiac loop participates functionally in the actomyosin interaction

Katalin Ajtai, Susanna P. Garamszegi, Shinya Watanabe, Mitsuo Ikebe, Thomas P. Burghardt

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The motor protein myosin in association with actin transduces chemical free energy in ATP into work in the form of actin translation against an opposing force. Mediating the actomyosin interaction in myosin is an actin binding site distributed among several peptides on the myosin surface including surface loops contributing to affinity and actin regulation of myosin ATPase. A structured surface loop on β-cardiac myosin, the cardiac or C-loop, was recently demonstrated to affect myosin ATPase and was indirectly implicated in the actomyosin interaction. The C-loop is a conserved feature of all myosin isoforms with crystal structures, suggesting that it is an essential part of the core energy transduction machinery. It is shown here that proteolytic digestion of the C-loop in β-cardiac myosin eliminates actin-activated myosin ATPase and reduces actomyosin affinity in rigor more than 100-fold. Studies of C-loop function in smooth muscle myosin were also undertaken using site-directed mutagenesis. Mutagenesis of a single charged residue in the C-loop of smooth muscle myosin alters actomyosin affinity and doubles myosin in vitro motility and actin-activated ATPase velocities, thereby involving a charged region of the loop in the actomyosin interaction. It appears likely that the C-loop is an essential electrostatic binding site for actin involved in modulation of actomyosin affinity and regulation of actomyosin ATPase velocity.

Original languageEnglish (US)
Pages (from-to)23415-23421
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number22
DOIs
StatePublished - May 28 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The myosin cardiac loop participates functionally in the actomyosin interaction'. Together they form a unique fingerprint.

  • Cite this

    Ajtai, K., Garamszegi, S. P., Watanabe, S., Ikebe, M., & Burghardt, T. P. (2004). The myosin cardiac loop participates functionally in the actomyosin interaction. Journal of Biological Chemistry, 279(22), 23415-23421. https://doi.org/10.1074/jbc.M310775200