The murine equivalent of the A181E TACI mutation associated with common variable immunodeficiency severely impairs B-cell function

John J. Lee, Ingrid Rauter, Lilit Garibyan, Esra Ozcan, Tatyana Sannikova, Stacey R. Dillon, Anthony C. Cruz, Richard M. Siegel, Richard J Bram, Haifa Jabara, Raif S. Geha

Research output: Contribution to journalArticle

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Abstract

TNFRSF13B, which encodes TACI (transmembrane activator and calcium-modulator and cyclophilin ligand interactor), is mutated in 10% of patients with common variable immune deficiency (CVID). One of the 2 most common TACI mutations in CVID, A181E, introduces a negative charge into the transmembrane domain. To define the consequence of the A181E mutation on TACI function, we studied the effect of its murine equivalent, mTACI A144E, on TACI signaling in transfected cells and on TACI function in transgenic mice. The mTACI A144E mutant, like its human TACI A181E counterpart, was expressed on the surface of 293T transfectants and was able to bind ligand, but exhibited impaired constitutive and ligand-induced NFκB signaling. In addition, constitutive and ligand-induced clustering of the intracellular domain was deficient for A144E as measured by fluorescence resonance energy transfer. Transgenic mice expressing the A144E mutant on TACI-/- background had low serum IgA levels and significantly impaired antibody responses to the type II T-independent antigen TNP-Ficoll. B cells from A144E transgenic mice were impaired in their capacity to proliferate and secrete IgG1 and IgA in response to TACI ligation. These results suggest thatmTACI A144E mutation and its human counterpart, A181E, disrupt TACI signaling and impair TACI-dependent B-cell functions.

Original languageEnglish (US)
Pages (from-to)2254-2262
Number of pages9
JournalBlood
Volume114
Issue number11
DOIs
StatePublished - 2009

Fingerprint

Common Variable Immunodeficiency
B-Lymphocytes
Cells
Transgenic Mice
Ligands
Mutation
Immunoglobulin A
T Independent Antigens
Cyclophilins
Fluorescence Resonance Energy Transfer
Modulators
Antibody Formation
Ligation
Cluster Analysis
Immunoglobulin G
Calcium
Antibodies
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Immunology
  • Hematology

Cite this

Lee, J. J., Rauter, I., Garibyan, L., Ozcan, E., Sannikova, T., Dillon, S. R., ... Geha, R. S. (2009). The murine equivalent of the A181E TACI mutation associated with common variable immunodeficiency severely impairs B-cell function. Blood, 114(11), 2254-2262. https://doi.org/10.1182/blood-2008-11-189720

The murine equivalent of the A181E TACI mutation associated with common variable immunodeficiency severely impairs B-cell function. / Lee, John J.; Rauter, Ingrid; Garibyan, Lilit; Ozcan, Esra; Sannikova, Tatyana; Dillon, Stacey R.; Cruz, Anthony C.; Siegel, Richard M.; Bram, Richard J; Jabara, Haifa; Geha, Raif S.

In: Blood, Vol. 114, No. 11, 2009, p. 2254-2262.

Research output: Contribution to journalArticle

Lee, JJ, Rauter, I, Garibyan, L, Ozcan, E, Sannikova, T, Dillon, SR, Cruz, AC, Siegel, RM, Bram, RJ, Jabara, H & Geha, RS 2009, 'The murine equivalent of the A181E TACI mutation associated with common variable immunodeficiency severely impairs B-cell function', Blood, vol. 114, no. 11, pp. 2254-2262. https://doi.org/10.1182/blood-2008-11-189720
Lee, John J. ; Rauter, Ingrid ; Garibyan, Lilit ; Ozcan, Esra ; Sannikova, Tatyana ; Dillon, Stacey R. ; Cruz, Anthony C. ; Siegel, Richard M. ; Bram, Richard J ; Jabara, Haifa ; Geha, Raif S. / The murine equivalent of the A181E TACI mutation associated with common variable immunodeficiency severely impairs B-cell function. In: Blood. 2009 ; Vol. 114, No. 11. pp. 2254-2262.
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abstract = "TNFRSF13B, which encodes TACI (transmembrane activator and calcium-modulator and cyclophilin ligand interactor), is mutated in 10{\%} of patients with common variable immune deficiency (CVID). One of the 2 most common TACI mutations in CVID, A181E, introduces a negative charge into the transmembrane domain. To define the consequence of the A181E mutation on TACI function, we studied the effect of its murine equivalent, mTACI A144E, on TACI signaling in transfected cells and on TACI function in transgenic mice. The mTACI A144E mutant, like its human TACI A181E counterpart, was expressed on the surface of 293T transfectants and was able to bind ligand, but exhibited impaired constitutive and ligand-induced NFκB signaling. In addition, constitutive and ligand-induced clustering of the intracellular domain was deficient for A144E as measured by fluorescence resonance energy transfer. Transgenic mice expressing the A144E mutant on TACI-/- background had low serum IgA levels and significantly impaired antibody responses to the type II T-independent antigen TNP-Ficoll. B cells from A144E transgenic mice were impaired in their capacity to proliferate and secrete IgG1 and IgA in response to TACI ligation. These results suggest thatmTACI A144E mutation and its human counterpart, A181E, disrupt TACI signaling and impair TACI-dependent B-cell functions.",
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