The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival

Doo Sup Choi, Hannah Young, Thomas McMahon, Dan Wang, Robert O. Messing

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.

Original languageEnglish (US)
Pages (from-to)1541-1548
Number of pages8
JournalMolecular pharmacology
Volume64
Issue number6
DOIs
StatePublished - Dec 1 2003

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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