The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival

Doo Sup Choi, Hannah Young, Thomas McMahon, Dan Wang, Robert O. Messing

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.

Original languageEnglish (US)
Pages (from-to)1541-1548
Number of pages8
JournalMolecular Pharmacology
Volume64
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Cell Survival
Nerve Growth Factor
Genes
Amino Acid Motifs
Serum-Free Culture Media
Cell Surface Receptors
Cytoplasmic and Nuclear Receptors
receptors for activated C kinase
Introns
Protein Kinases
Exons
Transcription Factors
Chromosomes
Gene Expression
Serum

ASJC Scopus subject areas

  • Pharmacology

Cite this

The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival. / Choi, Doo Sup; Young, Hannah; McMahon, Thomas; Wang, Dan; Messing, Robert O.

In: Molecular Pharmacology, Vol. 64, No. 6, 12.2003, p. 1541-1548.

Research output: Contribution to journalArticle

Choi, Doo Sup ; Young, Hannah ; McMahon, Thomas ; Wang, Dan ; Messing, Robert O. / The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival. In: Molecular Pharmacology. 2003 ; Vol. 64, No. 6. pp. 1541-1548.
@article{7e4c81aa69d34b3bbe93002702ff050a,
title = "The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival",
abstract = "Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.",
author = "Choi, {Doo Sup} and Hannah Young and Thomas McMahon and Dan Wang and Messing, {Robert O.}",
year = "2003",
month = "12",
doi = "10.1124/mol.64.6.1541",
language = "English (US)",
volume = "64",
pages = "1541--1548",
journal = "Molecular Pharmacology",
issn = "0026-895X",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "6",

}

TY - JOUR

T1 - The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival

AU - Choi, Doo Sup

AU - Young, Hannah

AU - McMahon, Thomas

AU - Wang, Dan

AU - Messing, Robert O.

PY - 2003/12

Y1 - 2003/12

N2 - Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.

AB - Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.

UR - http://www.scopus.com/inward/record.url?scp=0346366930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346366930&partnerID=8YFLogxK

U2 - 10.1124/mol.64.6.1541

DO - 10.1124/mol.64.6.1541

M3 - Article

C2 - 14645685

AN - SCOPUS:0346366930

VL - 64

SP - 1541

EP - 1548

JO - Molecular Pharmacology

JF - Molecular Pharmacology

SN - 0026-895X

IS - 6

ER -