The mouse androgen receptor gene contains a second functional promoter which is regulated by dihydrotestosterone

M. E. Grossmann, J. Lindzey, L. Blok, J. E. Perry, M. V. Kumar, D. J. Tindall

Research output: Contribution to journalArticle

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Abstract

The androgen receptor (AR) is a developmental and tissue-specific transcription factor which is activated by binding testosterone or dihydrotestosterone. Several different methods of transcriptional regulation of the AR have been shown, including regulation by androgens, follicle- stimulating hormone, epidermal growth factor, and the cAMP pathway. In order to further characterize the transcriptional regulation of the AR, portions of the mouse androgen receptor (mAR) promoter were cloned into the promoterless pBLCAT3 vector and assayed for chloramphenicol acetyltransferase activity. The results indicate that in addition to the previously characterized promoter (+1) there is a second distinct promoter located 3' to the first promoter. Amplification of the 5'-end of the AR gene indicates that RNA originating from the second promoter is initiated from 162 and 170 bases downstream from the 5'-most previously characterized site. Northern blot analysis indicated that RNA initiated from the two promoters is differentially expressed in several cell lines and multiple tissues. Androgen ablation by castration showed that both promoters are controlled by androgens in the kidney. Sequence analysis revealed that the second promoter does not contain a TATA or CAAT box. Further characterization of this promoter may provide important insights into the transcriptional regulation of the androgen receptor since previous studies have often included only the first promoter.

Original languageEnglish (US)
Pages (from-to)14594-14600
Number of pages7
JournalBiochemistry
Volume33
Issue number48
DOIs
StatePublished - 1994

Fingerprint

Dihydrotestosterone
Androgen Receptors
Genes
Androgens
RNA
Chloramphenicol O-Acetyltransferase
Tissue
Castration
Follicle Stimulating Hormone
Epidermal Growth Factor
Northern Blotting
Sequence Analysis
Testosterone
Ablation
Transcription Factors
Amplification
mouse AR protein
Kidney
Cell Line
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Grossmann, M. E., Lindzey, J., Blok, L., Perry, J. E., Kumar, M. V., & Tindall, D. J. (1994). The mouse androgen receptor gene contains a second functional promoter which is regulated by dihydrotestosterone. Biochemistry, 33(48), 14594-14600. https://doi.org/10.1021/bi00252a027

The mouse androgen receptor gene contains a second functional promoter which is regulated by dihydrotestosterone. / Grossmann, M. E.; Lindzey, J.; Blok, L.; Perry, J. E.; Kumar, M. V.; Tindall, D. J.

In: Biochemistry, Vol. 33, No. 48, 1994, p. 14594-14600.

Research output: Contribution to journalArticle

Grossmann, ME, Lindzey, J, Blok, L, Perry, JE, Kumar, MV & Tindall, DJ 1994, 'The mouse androgen receptor gene contains a second functional promoter which is regulated by dihydrotestosterone', Biochemistry, vol. 33, no. 48, pp. 14594-14600. https://doi.org/10.1021/bi00252a027
Grossmann, M. E. ; Lindzey, J. ; Blok, L. ; Perry, J. E. ; Kumar, M. V. ; Tindall, D. J. / The mouse androgen receptor gene contains a second functional promoter which is regulated by dihydrotestosterone. In: Biochemistry. 1994 ; Vol. 33, No. 48. pp. 14594-14600.
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