TY - JOUR
T1 - The mitotic kinesin CENP-E is a processive transport motor
AU - Yardimci, Hasan
AU - Van Duffelen, Marilyn
AU - Mao, Yinghui
AU - Rosenfeld, Steven S.
AU - Selvin, Paul R.
PY - 2008/4/22
Y1 - 2008/4/22
N2 - In vivo studies suggest that centromeric protein E (CENP-E), a kinesin-7 family member, plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. How CENP-E accomplishes this crucial task, however, is not clear. Here we present single-molecule measurements of CENP-E that demonstrate that this motor moves processively toward the plus end of microtubules, with an average run length of 2.6 ± 0.2 μm, in a hand-over-hand fashion, taking 8-nm steps with a stall force of 6 ± 0.1 pN. The ATP dependence of motor velocity obeys Michaelis-Menten kinetics with KM,ATP = 35 ± 5 μM. All of these features are remarkably similar to those for kinesin-1 - a highly processive transport motor. We, therefore, propose that CENP-E transports chromosomes in a manner analogous to how kinesin-1 transports cytoplasmic vesicles.
AB - In vivo studies suggest that centromeric protein E (CENP-E), a kinesin-7 family member, plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. How CENP-E accomplishes this crucial task, however, is not clear. Here we present single-molecule measurements of CENP-E that demonstrate that this motor moves processively toward the plus end of microtubules, with an average run length of 2.6 ± 0.2 μm, in a hand-over-hand fashion, taking 8-nm steps with a stall force of 6 ± 0.1 pN. The ATP dependence of motor velocity obeys Michaelis-Menten kinetics with KM,ATP = 35 ± 5 μM. All of these features are remarkably similar to those for kinesin-1 - a highly processive transport motor. We, therefore, propose that CENP-E transports chromosomes in a manner analogous to how kinesin-1 transports cytoplasmic vesicles.
KW - Mitotic motor
KW - Single molecule
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U2 - 10.1073/pnas.0711314105
DO - 10.1073/pnas.0711314105
M3 - Article
C2 - 18427114
AN - SCOPUS:43149115167
SN - 0027-8424
VL - 105
SP - 6016
EP - 6021
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -