The Metabolic Syndrome Does Not Affect Development of Collateral Circulation in the Poststenotic Swine Kidney

Xin Zhang, Seo Rin Kim, Christopher M. Ferguson, Behzad Ebrahimi, Ahmad F. Hedayat, Amir Lerman, Lilach O Lerman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND: The collateral circulation is important in maintenance of blood supply to the ischemic kidney distal to renal artery stenosis (RAS). Obesity metabolic syndrome (MetS) preserves renal blood flow (RBF) in the stenotic kidney, but whether this is related to an increase of collateral vessel growth is unknown. We hypothesized that MetS increased collateral circulation around the renal artery. METHODS: Twenty-one domestic pigs were randomly divided into unilateral RAS fed an atherogenic (high-fat/high-fructose, MetS-RAS) or standard diet, or controls (n = 7 each). RBF, glomerular filtration rate (GFR), and the peristenotic collateral circulation were assessed after 10 weeks using multidetector computed tomography (CT) and the intrarenal microcirculation by micro-CT. Vascular endothelial growth factor (VEGF) expression was studied in the renal artery wall, kidney, and perirenal fat. Renal fibrosis and stiffness were examined by trichrome and magnetic resonance elastography. RESULTS: Compared with controls, RBF and GFR were decreased in RAS, but not in MetS-RAS. MetS-RAS formed peristenotic collaterals to the same extent as RAS pigs but induced greater intrarenal microvascular loss, fibrosis, stiffness, and inflammation. MetS-RAS also attenuated VEGF expression in the renal tissue compared with RAS, despite increased expression in the perirenal fat. CONCLUSIONS: MetS does not interfere with collateral vessel formation in the stenotic kidney, possibly because decreased renal arterial VEGF expression offsets its upregulation in perirenal fat, arguing against a major contribution of the collateral circulation to preserve renal function in MetS-RAS. Furthermore, preserved renal function does not protect the poststenotic kidney from parenchymal injury.

Original languageEnglish (US)
Pages (from-to)1307-1316
Number of pages10
JournalAmerican Journal of Hypertension
Volume31
Issue number12
DOIs
StatePublished - Nov 13 2018

Fingerprint

Collateral Circulation
Renal Artery Obstruction
Swine
Kidney
Renal Circulation
Fats
Vascular Endothelial Growth Factor A
Renal Artery
Glomerular Filtration Rate
Fibrosis
Elasticity Imaging Techniques
Sus scrofa
Multidetector Computed Tomography
Microcirculation
Fructose
Up-Regulation
Obesity
Tomography
Maintenance
Diet

ASJC Scopus subject areas

  • Internal Medicine

Cite this

The Metabolic Syndrome Does Not Affect Development of Collateral Circulation in the Poststenotic Swine Kidney. / Zhang, Xin; Kim, Seo Rin; Ferguson, Christopher M.; Ebrahimi, Behzad; Hedayat, Ahmad F.; Lerman, Amir; Lerman, Lilach O.

In: American Journal of Hypertension, Vol. 31, No. 12, 13.11.2018, p. 1307-1316.

Research output: Contribution to journalArticle

Zhang, Xin ; Kim, Seo Rin ; Ferguson, Christopher M. ; Ebrahimi, Behzad ; Hedayat, Ahmad F. ; Lerman, Amir ; Lerman, Lilach O. / The Metabolic Syndrome Does Not Affect Development of Collateral Circulation in the Poststenotic Swine Kidney. In: American Journal of Hypertension. 2018 ; Vol. 31, No. 12. pp. 1307-1316.
@article{b94613a4e6244c03af322a1d09147c5d,
title = "The Metabolic Syndrome Does Not Affect Development of Collateral Circulation in the Poststenotic Swine Kidney",
abstract = "BACKGROUND: The collateral circulation is important in maintenance of blood supply to the ischemic kidney distal to renal artery stenosis (RAS). Obesity metabolic syndrome (MetS) preserves renal blood flow (RBF) in the stenotic kidney, but whether this is related to an increase of collateral vessel growth is unknown. We hypothesized that MetS increased collateral circulation around the renal artery. METHODS: Twenty-one domestic pigs were randomly divided into unilateral RAS fed an atherogenic (high-fat/high-fructose, MetS-RAS) or standard diet, or controls (n = 7 each). RBF, glomerular filtration rate (GFR), and the peristenotic collateral circulation were assessed after 10 weeks using multidetector computed tomography (CT) and the intrarenal microcirculation by micro-CT. Vascular endothelial growth factor (VEGF) expression was studied in the renal artery wall, kidney, and perirenal fat. Renal fibrosis and stiffness were examined by trichrome and magnetic resonance elastography. RESULTS: Compared with controls, RBF and GFR were decreased in RAS, but not in MetS-RAS. MetS-RAS formed peristenotic collaterals to the same extent as RAS pigs but induced greater intrarenal microvascular loss, fibrosis, stiffness, and inflammation. MetS-RAS also attenuated VEGF expression in the renal tissue compared with RAS, despite increased expression in the perirenal fat. CONCLUSIONS: MetS does not interfere with collateral vessel formation in the stenotic kidney, possibly because decreased renal arterial VEGF expression offsets its upregulation in perirenal fat, arguing against a major contribution of the collateral circulation to preserve renal function in MetS-RAS. Furthermore, preserved renal function does not protect the poststenotic kidney from parenchymal injury.",
author = "Xin Zhang and Kim, {Seo Rin} and Ferguson, {Christopher M.} and Behzad Ebrahimi and Hedayat, {Ahmad F.} and Amir Lerman and Lerman, {Lilach O}",
year = "2018",
month = "11",
day = "13",
doi = "10.1093/ajh/hpy127",
language = "English (US)",
volume = "31",
pages = "1307--1316",
journal = "American Journal of Hypertension",
issn = "0895-7061",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - The Metabolic Syndrome Does Not Affect Development of Collateral Circulation in the Poststenotic Swine Kidney

AU - Zhang, Xin

AU - Kim, Seo Rin

AU - Ferguson, Christopher M.

AU - Ebrahimi, Behzad

AU - Hedayat, Ahmad F.

AU - Lerman, Amir

AU - Lerman, Lilach O

PY - 2018/11/13

Y1 - 2018/11/13

N2 - BACKGROUND: The collateral circulation is important in maintenance of blood supply to the ischemic kidney distal to renal artery stenosis (RAS). Obesity metabolic syndrome (MetS) preserves renal blood flow (RBF) in the stenotic kidney, but whether this is related to an increase of collateral vessel growth is unknown. We hypothesized that MetS increased collateral circulation around the renal artery. METHODS: Twenty-one domestic pigs were randomly divided into unilateral RAS fed an atherogenic (high-fat/high-fructose, MetS-RAS) or standard diet, or controls (n = 7 each). RBF, glomerular filtration rate (GFR), and the peristenotic collateral circulation were assessed after 10 weeks using multidetector computed tomography (CT) and the intrarenal microcirculation by micro-CT. Vascular endothelial growth factor (VEGF) expression was studied in the renal artery wall, kidney, and perirenal fat. Renal fibrosis and stiffness were examined by trichrome and magnetic resonance elastography. RESULTS: Compared with controls, RBF and GFR were decreased in RAS, but not in MetS-RAS. MetS-RAS formed peristenotic collaterals to the same extent as RAS pigs but induced greater intrarenal microvascular loss, fibrosis, stiffness, and inflammation. MetS-RAS also attenuated VEGF expression in the renal tissue compared with RAS, despite increased expression in the perirenal fat. CONCLUSIONS: MetS does not interfere with collateral vessel formation in the stenotic kidney, possibly because decreased renal arterial VEGF expression offsets its upregulation in perirenal fat, arguing against a major contribution of the collateral circulation to preserve renal function in MetS-RAS. Furthermore, preserved renal function does not protect the poststenotic kidney from parenchymal injury.

AB - BACKGROUND: The collateral circulation is important in maintenance of blood supply to the ischemic kidney distal to renal artery stenosis (RAS). Obesity metabolic syndrome (MetS) preserves renal blood flow (RBF) in the stenotic kidney, but whether this is related to an increase of collateral vessel growth is unknown. We hypothesized that MetS increased collateral circulation around the renal artery. METHODS: Twenty-one domestic pigs were randomly divided into unilateral RAS fed an atherogenic (high-fat/high-fructose, MetS-RAS) or standard diet, or controls (n = 7 each). RBF, glomerular filtration rate (GFR), and the peristenotic collateral circulation were assessed after 10 weeks using multidetector computed tomography (CT) and the intrarenal microcirculation by micro-CT. Vascular endothelial growth factor (VEGF) expression was studied in the renal artery wall, kidney, and perirenal fat. Renal fibrosis and stiffness were examined by trichrome and magnetic resonance elastography. RESULTS: Compared with controls, RBF and GFR were decreased in RAS, but not in MetS-RAS. MetS-RAS formed peristenotic collaterals to the same extent as RAS pigs but induced greater intrarenal microvascular loss, fibrosis, stiffness, and inflammation. MetS-RAS also attenuated VEGF expression in the renal tissue compared with RAS, despite increased expression in the perirenal fat. CONCLUSIONS: MetS does not interfere with collateral vessel formation in the stenotic kidney, possibly because decreased renal arterial VEGF expression offsets its upregulation in perirenal fat, arguing against a major contribution of the collateral circulation to preserve renal function in MetS-RAS. Furthermore, preserved renal function does not protect the poststenotic kidney from parenchymal injury.

UR - http://www.scopus.com/inward/record.url?scp=85056536635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056536635&partnerID=8YFLogxK

U2 - 10.1093/ajh/hpy127

DO - 10.1093/ajh/hpy127

M3 - Article

VL - 31

SP - 1307

EP - 1316

JO - American Journal of Hypertension

JF - American Journal of Hypertension

SN - 0895-7061

IS - 12

ER -