The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins

James H. Thierer, Stephen C Ekker, Steven A. Farber

Research output: Contribution to journalArticle

Abstract

Apolipoprotein-B (ApoB) is the structural component of atherogenic lipoproteins, lipid-rich particles that drive atherosclerosis by accumulating in the vascular wall. As atherosclerotic cardiovascular disease is the leading cause of death worldwide, there is an urgent need to develop new strategies to prevent lipoproteins from causing vascular damage. Here we report the LipoGlo system, which uses a luciferase enzyme (NanoLuc) fused to ApoB to monitor several key determinants of lipoprotein atherogenicity including particle abundance, size, and localization. Using LipoGlo, we comprehensively characterize the lipoprotein profile of individual larval zebrafish and collect images of atherogenic lipoprotein localization in an intact organism. We report multiple extravascular lipoprotein localization patterns, as well as identify Pla2g12b as a potent regulator of lipoprotein size. ApoB-fusion proteins thus represent a sensitive and specific approach to study atherogenic lipoproteins and their genetic and small molecule modifiers.

Original languageEnglish (US)
Number of pages1
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Jul 31 2019

Fingerprint

lipoproteins
Lipoproteins
Monitoring
Apolipoproteins B
Blood Vessels
arteriosclerosis
regulators
Zebrafish
Luciferases
organisms
Particle Size
death
determinants
lipids
enzymes
Cause of Death
Atherosclerosis
Cardiovascular Diseases
Fusion reactions
fusion

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. / Thierer, James H.; Ekker, Stephen C; Farber, Steven A.

In: Nature communications, Vol. 10, No. 1, 31.07.2019.

Research output: Contribution to journalArticle

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