The lac operator-repressor system is functional in the mouse

C. A. Cronin, W. Gluba, H. Scrable

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

We report the successful transfer of a fully functional lac operator-repressor gene regulatory system to the mouse. The key component is a lac repressor transgene that resembles a typical mammalian gene both in codon usage and structure and expresses functional levels of repressor protein in the animal. We used the repressor to regulate the expression of a mammalian reporter gene consisting of the tyrosinase promoter embedded with three short lac operator sequences and the tyrosinase coding sequence. Pigmentation of the mouse was controlled by the interaction of the lac repressor with the regulatable Tyrosinase transgene in a manner that was fully reversible by the lactose analog IPTG. Direct control of mammalian promoters by the lac repressor provides tight, reversible regulation, predictable levels of de-repressed expression, and the promise of reversible control of the endogenous genome.

Original languageEnglish (US)
Pages (from-to)1506-1517
Number of pages12
JournalGenes and Development
Volume15
Issue number12
DOIs
StatePublished - Jun 15 2001
Externally publishedYes

Fingerprint

Lac Repressors
Monophenol Monooxygenase
Transgenes
Repressor Proteins
Isopropyl Thiogalactoside
Pigmentation
Lactose
Regulator Genes
Reporter Genes
Codon
Genome
Genes

Keywords

  • Albino
  • Gene regulation
  • lac repressor
  • Pigmentation
  • Transgenic mice
  • Tyrosinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The lac operator-repressor system is functional in the mouse. / Cronin, C. A.; Gluba, W.; Scrable, H.

In: Genes and Development, Vol. 15, No. 12, 15.06.2001, p. 1506-1517.

Research output: Contribution to journalArticle

Cronin, CA, Gluba, W & Scrable, H 2001, 'The lac operator-repressor system is functional in the mouse', Genes and Development, vol. 15, no. 12, pp. 1506-1517. https://doi.org/10.1101/gad.892001
Cronin, C. A. ; Gluba, W. ; Scrable, H. / The lac operator-repressor system is functional in the mouse. In: Genes and Development. 2001 ; Vol. 15, No. 12. pp. 1506-1517.
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