The lac operator-repressor system is functional in the mouse

C. A. Cronin, W. Gluba, H. Scrable

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

We report the successful transfer of a fully functional lac operator-repressor gene regulatory system to the mouse. The key component is a lac repressor transgene that resembles a typical mammalian gene both in codon usage and structure and expresses functional levels of repressor protein in the animal. We used the repressor to regulate the expression of a mammalian reporter gene consisting of the tyrosinase promoter embedded with three short lac operator sequences and the tyrosinase coding sequence. Pigmentation of the mouse was controlled by the interaction of the lac repressor with the regulatable Tyrosinase transgene in a manner that was fully reversible by the lactose analog IPTG. Direct control of mammalian promoters by the lac repressor provides tight, reversible regulation, predictable levels of de-repressed expression, and the promise of reversible control of the endogenous genome.

Original languageEnglish (US)
Pages (from-to)1506-1517
Number of pages12
JournalGenes and Development
Volume15
Issue number12
DOIs
StatePublished - Jun 15 2001

Keywords

  • Albino
  • Gene regulation
  • Pigmentation
  • Transgenic mice
  • Tyrosinase
  • lac repressor

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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