Abstract
An association between an activating JAK2 mutation (JAK2V617F) and BCR/ABL-negative myeloproliferative disorders was recently reported in multiple simultaneous publications. In the current study, mutation analysis for JAK2V617F was performed in peripheral blood mononuclear cells (PBMC) from 157 patients with myelofibrosis with myeloid metaplasia (MMM) including 117 with agnogenic (AMM), 22 with postpolycythaemic (PPMM), and 18 with post-thrombocythaemic (PTMM) myeloid metaplasia. The detection rate for JAK2V617F was significantly higher in PPMM (91%; homozygous in 18%) compared with either AMM (45.3%; homozygous in 2.6%) or PTMM (38.9%; homozygous in 11.1%). Concomitant analysis in granulocytes (n = 57) and CD34+ cells (n = 25) disclosed a higher incidence of homozygous JAK2V617F mutation but the overall mutation rate was similar to that obtained from PBMC. JAK2V617F was not detected in DNA derived from T cells (n = 19). In AMM, the presence of JAK2V617F was associated with an older age at diagnosis and a history of thrombosis or pruritus. Multivariate analysis identified only age and the Dupriez prognostic score as independent prognostic factors; JAK2V617F had no prognostic significance. In conclusion, JAK2V617F is a myeloid lineage-specific event, its incidence in MMM is significantly higher with an antecedent history of polycythaemia vera (PV), and its presence in AMM does not affect prognosis but is associated with PV-characteristic clinical features.
Original language | English (US) |
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Pages (from-to) | 320-328 |
Number of pages | 9 |
Journal | British journal of haematology |
Volume | 131 |
Issue number | 3 |
DOIs | |
State | Published - Nov 2005 |
Keywords
- Clonality
- JAK2
- Mutation
- Myelofibrosis
- Polycythaemia vera
- Prognosis
ASJC Scopus subject areas
- Hematology