The Isolation and Partial Characterization of a New Restriction Endonuclease from Providencia Stuartii

David L. Smith, Frederick R. Blattner, Julian Davies

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.

Original languageEnglish (US)
Pages (from-to)343-354
Number of pages12
JournalNucleic acids research
Volume3
Issue number2
DOIs
StatePublished - Feb 1976

ASJC Scopus subject areas

  • Genetics

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