The Isolation and Partial Characterization of a New Restriction Endonuclease from Providencia Stuartii

David L. Smith; Frederick R. Blattner; Julian Davies

doi:10.1093/nar/3.2.343

The Isolation and Partial Characterization of a New Restriction Endonuclease from Providencia Stuartii

David L. Smith, Frederick R. Blattner, Julian Davies

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg⁺⁺ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.

Original language	English (US)
Pages (from-to)	343-354
Number of pages	12
Journal	Nucleic acids research
Volume	3
Issue number	2
DOIs	https://doi.org/10.1093/nar/3.2.343
State	Published - Feb 1976

ASJC Scopus subject areas

Genetics

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title = "The Isolation and Partial Characterization of a New Restriction Endonuclease from Providencia Stuartii",

abstract = "We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.",

author = "Smith, {David L.} and Blattner, {Frederick R.} and Julian Davies",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by an NSF grant #BMS72-02264. The authors wish to thank Dr. B. Weisblum for much useful advice and encouragement and also Dr. L. Crawford and Dr. R. Roberts for sharing their knowledge of restriction endonucleases.",

year = "1976",

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AU - Blattner, Frederick R.

AU - Davies, Julian

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PY - 1976/2

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AB - We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.

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The Isolation and Partial Characterization of a New Restriction Endonuclease from Providencia Stuartii

Abstract

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