The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing

Angela Miele, Ricardo Medina, Andre J van Wijnen, Gary S. Stein, Janet L. Stein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220NPAT to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation.

Original languageEnglish (US)
Pages (from-to)136-148
Number of pages13
JournalJournal of Cellular Biochemistry
Volume102
Issue number1
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Regulator Genes
Histones
Cell Cycle
Genes
Cells
RNA
Processing
Cyclin E
Transcription
Proteins
Yeast
Yeasts
Protein Splicing
Phase Transition
G1 Phase
Cell Cycle Checkpoints
S Phase
Immunoprecipitation
Genetic Promoter Regions
Gene expression

Keywords

  • Cajal body
  • CDK2
  • Cell cycle
  • Cyclin
  • Histone
  • RNA processing
  • Transcription
  • Zn finger

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing. / Miele, Angela; Medina, Ricardo; van Wijnen, Andre J; Stein, Gary S.; Stein, Janet L.

In: Journal of Cellular Biochemistry, Vol. 102, No. 1, 01.09.2007, p. 136-148.

Research output: Contribution to journalArticle

Miele, Angela ; Medina, Ricardo ; van Wijnen, Andre J ; Stein, Gary S. ; Stein, Janet L. / The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing. In: Journal of Cellular Biochemistry. 2007 ; Vol. 102, No. 1. pp. 136-148.
@article{5d40794bdcb54200821bed772f9866cd,
title = "The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing",
abstract = "HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220NPAT to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation.",
keywords = "Cajal body, CDK2, Cell cycle, Cyclin, Histone, RNA processing, Transcription, Zn finger",
author = "Angela Miele and Ricardo Medina and {van Wijnen}, {Andre J} and Stein, {Gary S.} and Stein, {Janet L.}",
year = "2007",
month = "9",
day = "1",
doi = "10.1002/jcb.21284",
language = "English (US)",
volume = "102",
pages = "136--148",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing

AU - Miele, Angela

AU - Medina, Ricardo

AU - van Wijnen, Andre J

AU - Stein, Gary S.

AU - Stein, Janet L.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220NPAT to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation.

AB - HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220NPAT to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation.

KW - Cajal body

KW - CDK2

KW - Cell cycle

KW - Cyclin

KW - Histone

KW - RNA processing

KW - Transcription

KW - Zn finger

UR - http://www.scopus.com/inward/record.url?scp=34548509438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548509438&partnerID=8YFLogxK

U2 - 10.1002/jcb.21284

DO - 10.1002/jcb.21284

M3 - Article

C2 - 17577209

AN - SCOPUS:34548509438

VL - 102

SP - 136

EP - 148

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 1

ER -