The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC

Jun Zhong, Raghothama Chaerkady, Kumaran Kandasamy, Marjan Gucek, Robert N. Cole, Akhilesh Pandey

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Signal transduction pathways are tightly controlled by positive and negative regulators. We have previously identified Odin (also known as ankyrin repeat and sterile alpha motif domain-containing 1A; gene symbol ANKS1A) as a negative regulator of growth factor signaling; however, the mechanisms through which Odin regulates these pathways remain to be elucidated. To determine how Odin negatively regulates growth factor signaling, we undertook a proteomic approach to systematically identify proteins that interact with Odin using the SILAC strategy. In this study, we identified 18 molecules that were specifically associated in a protein complex with Odin. Our study established that the complete family of 14-3-3 proteins occur in a protein complex with Odin, which is also supported by earlier reports that identified a few members of the 14-3-3 family as Odin interactors. Among the novel protein interactors of Odin were CD2-associated protein, SH3 domain kinase binding protein 1 and DAB2 interacting protein. We confirmed 8 of the eighteen interactions identified in the Odin protein complex by co-immunoprecipitation experiments. Finally, a literature-based network analysis revealed that Odin interacting partners are involved in various cellular processes, some of which are key molecules in regulating receptor endocytosis.

Original languageEnglish (US)
Pages (from-to)294-303
Number of pages10
JournalJournal of Proteomics
Volume74
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Fingerprint

Proteins
Intercellular Signaling Peptides and Proteins
Ankyrin Repeat
14-3-3 Proteins
Signal transduction
Molecules
src Homology Domains
Electric network analysis
Endocytosis
Immunoprecipitation
Proteomics
Signal Transduction
Carrier Proteins
Phosphotransferases
Genes
Experiments
Sterile Alpha Motif
CD2-associated protein
Protein Domains

Keywords

  • Interacting proteins
  • Odin (ANKS1A)
  • Receptor tyrosine kinase
  • SILAC

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC. / Zhong, Jun; Chaerkady, Raghothama; Kandasamy, Kumaran; Gucek, Marjan; Cole, Robert N.; Pandey, Akhilesh.

In: Journal of Proteomics, Vol. 74, No. 3, 01.03.2011, p. 294-303.

Research output: Contribution to journalArticle

Zhong, Jun ; Chaerkady, Raghothama ; Kandasamy, Kumaran ; Gucek, Marjan ; Cole, Robert N. ; Pandey, Akhilesh. / The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC. In: Journal of Proteomics. 2011 ; Vol. 74, No. 3. pp. 294-303.
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