The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: An in vitro study

V. J. Silvaggio; F. H. Fu; H. I. Georgescu; C. H. Evans

doi:10.1016/S0749-8063(05)80349-4

The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: An in vitro study

V. J. Silvaggio, F. H. Fu, H. I. Georgescu, C. H. Evans

Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxidetreated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovialconditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.

Original language	English (US)
Pages (from-to)	82-86
Number of pages	5
Journal	Arthroscopy
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/S0749-8063(05)80349-4
State	Published - 1993

Keywords

Anterior cruciate ligament
Ethylene oxide
Interleukin-1

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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10.1016/S0749-8063(05)80349-4

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@article{7024a27da5e14a3bac95ffa99b1e366a,

title = "The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: An in vitro study",

abstract = "There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxidetreated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovialconditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.",

keywords = "Anterior cruciate ligament, Ethylene oxide, Interleukin-1",

author = "Silvaggio, {V. J.} and Fu, {F. H.} and Georgescu, {H. I.} and Evans, {C. H.}",

note = "Funding Information: Acknowledgment: We thank Lori Mclntyre for performing the IL-1 assays. We also thank Dr, G. Klinzing and Mr. He of the University of Pittsburgh, Department of Chemical Engineering, for their help with the particle sizing. Our deep appreciation goes to Lou Duerring for her help in preparation of this article. Research was supported in part by the Presbyterian University Hospital Competitive Medical Research Grant and by a grant from the Ferguson Foundation.",

year = "1993",

doi = "10.1016/S0749-8063(05)80349-4",

language = "English (US)",

volume = "9",

pages = "82--86",

journal = "Arthroscopy",

issn = "0749-8063",

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T1 - The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles

T2 - An in vitro study

AU - Silvaggio, V. J.

AU - Fu, F. H.

AU - Georgescu, H. I.

AU - Evans, C. H.

N1 - Funding Information: Acknowledgment: We thank Lori Mclntyre for performing the IL-1 assays. We also thank Dr, G. Klinzing and Mr. He of the University of Pittsburgh, Department of Chemical Engineering, for their help with the particle sizing. Our deep appreciation goes to Lou Duerring for her help in preparation of this article. Research was supported in part by the Presbyterian University Hospital Competitive Medical Research Grant and by a grant from the Ferguson Foundation.

PY - 1993

Y1 - 1993

N2 - There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxidetreated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovialconditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.

AB - There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxidetreated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovialconditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.

KW - Anterior cruciate ligament

KW - Ethylene oxide

KW - Interleukin-1

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The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: An in vitro study

Abstract

Keywords

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