The identification of HLA class II-restricted T cell epitopes to vaccinia virus membrane proteins

Richard B. Kennedy, Gregory A. Poland

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Three decades after the eradication of smallpox, the threat of bioterrorism and outbreaks of emerging diseases such as monkeypox have renewed interest in the development of safe and effective next-generation poxvirus vaccines and biodefense research. Current smallpox vaccines contain live virus and are contraindicated for a large percentage of the population. Safer, yet still effective inactivated and subunit vaccines are needed, and epitope identification is an essential step in the development of these subunit vaccines. In this study we focused on 4 vaccinia membrane proteins known to be targeted by humoral responses in vaccinees. In spite of the narrow focus of the study we identified 36. T cell epitopes, and provide additional support for the physical linkage between T and B epitopes. This information may prove useful in peptide and protein-based subunit vaccine development as well as in the study of CD4 responses to poxviruses.

Original languageEnglish (US)
Pages (from-to)232-240
Number of pages9
JournalVirology
Volume408
Issue number2
DOIs
StatePublished - Dec 20 2010

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Keywords

  • CD4+ T cells
  • Cellular immunity
  • Smallpox vaccine
  • T cell epitopes
  • Vaccinia virus

ASJC Scopus subject areas

  • Virology

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