The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg)

T. Werfel, M. Oppermann, J. H. Butterfield, G. Begemann, J. Elsner, O. Götze, J. Zwirner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The expression of the receptor for the anaphylatoxin C5a (C5aR, CD88) on the human mast cell line HMC-1 was studied with four anti-C5aR monoclonal antibodies directed to the N-terminal domain of the receptor. All antibodies bound to the human mart cell line HMC-1. The binding could be blocked by recombinant C5a and by peptide EX-1 representing amino residues 1-31 on the N-terminal domain of the C5aR. In addition, FITC-labelled C5a bound to HMC-1, and this binding could be blocked by unlabelled C5a or C5aR antibodies. C5aR-specific mRNA was detected in HMC-1 cells by RT-PCR which confirmed the expression of the C5aR gene made by these cells. Lymphocyte-conditioned medium, interferon-γ or phorbol esters which have been shown to induce a down-regulation of C5aR On myeloid cells did not influence the expression of C5aR on HMC-1. C5a let to a transient mobilization of intracellular calcium in HMC-1 which could be inhibited by preincubation of C5a with a C5a-specific antibody. In contrast to findings with granulocytes, HMC-1 did not respond to C5a(desArg), confirming previous findings with human skin mast cells. The findings show that (i) although HMC-1 differ from granulocytes in their responsiveness to C5a(desArg), they express similar C5aR and (ii) HMC-1 resemble skin mast cells in the expression and function of C5aR and may therefore serve as a model in future studies addressing the biology of this anaphylatoxin receptor on skin mast cells.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalScandinavian Journal of Immunology
Volume44
Issue number1
StatePublished - 1996

Fingerprint

Anaphylatoxin C5a Receptor
Mast Cells
des-Arginine Complement C5a
Cell Line
Granulocytes
Skin
Antibodies
Anaphylatoxins
Fluorescein-5-isothiocyanate
Phorbol Esters
Myeloid Cells
Conditioned Culture Medium
Interferons
Down-Regulation
Monoclonal Antibodies
Lymphocytes
Calcium
Gene Expression
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Werfel, T., Oppermann, M., Butterfield, J. H., Begemann, G., Elsner, J., Götze, O., & Zwirner, J. (1996). The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg). Scandinavian Journal of Immunology, 44(1), 30-36.

The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg). / Werfel, T.; Oppermann, M.; Butterfield, J. H.; Begemann, G.; Elsner, J.; Götze, O.; Zwirner, J.

In: Scandinavian Journal of Immunology, Vol. 44, No. 1, 1996, p. 30-36.

Research output: Contribution to journalArticle

Werfel, T, Oppermann, M, Butterfield, JH, Begemann, G, Elsner, J, Götze, O & Zwirner, J 1996, 'The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg)', Scandinavian Journal of Immunology, vol. 44, no. 1, pp. 30-36.
Werfel T, Oppermann M, Butterfield JH, Begemann G, Elsner J, Götze O et al. The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg). Scandinavian Journal of Immunology. 1996;44(1):30-36.
Werfel, T. ; Oppermann, M. ; Butterfield, J. H. ; Begemann, G. ; Elsner, J. ; Götze, O. ; Zwirner, J. / The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg). In: Scandinavian Journal of Immunology. 1996 ; Vol. 44, No. 1. pp. 30-36.
@article{05812bc531c64ca7a448e113a4fb9e37,
title = "The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg)",
abstract = "The expression of the receptor for the anaphylatoxin C5a (C5aR, CD88) on the human mast cell line HMC-1 was studied with four anti-C5aR monoclonal antibodies directed to the N-terminal domain of the receptor. All antibodies bound to the human mart cell line HMC-1. The binding could be blocked by recombinant C5a and by peptide EX-1 representing amino residues 1-31 on the N-terminal domain of the C5aR. In addition, FITC-labelled C5a bound to HMC-1, and this binding could be blocked by unlabelled C5a or C5aR antibodies. C5aR-specific mRNA was detected in HMC-1 cells by RT-PCR which confirmed the expression of the C5aR gene made by these cells. Lymphocyte-conditioned medium, interferon-γ or phorbol esters which have been shown to induce a down-regulation of C5aR On myeloid cells did not influence the expression of C5aR on HMC-1. C5a let to a transient mobilization of intracellular calcium in HMC-1 which could be inhibited by preincubation of C5a with a C5a-specific antibody. In contrast to findings with granulocytes, HMC-1 did not respond to C5a(desArg), confirming previous findings with human skin mast cells. The findings show that (i) although HMC-1 differ from granulocytes in their responsiveness to C5a(desArg), they express similar C5aR and (ii) HMC-1 resemble skin mast cells in the expression and function of C5aR and may therefore serve as a model in future studies addressing the biology of this anaphylatoxin receptor on skin mast cells.",
author = "T. Werfel and M. Oppermann and Butterfield, {J. H.} and G. Begemann and J. Elsner and O. G{\"o}tze and J. Zwirner",
year = "1996",
language = "English (US)",
volume = "44",
pages = "30--36",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg)

AU - Werfel, T.

AU - Oppermann, M.

AU - Butterfield, J. H.

AU - Begemann, G.

AU - Elsner, J.

AU - Götze, O.

AU - Zwirner, J.

PY - 1996

Y1 - 1996

N2 - The expression of the receptor for the anaphylatoxin C5a (C5aR, CD88) on the human mast cell line HMC-1 was studied with four anti-C5aR monoclonal antibodies directed to the N-terminal domain of the receptor. All antibodies bound to the human mart cell line HMC-1. The binding could be blocked by recombinant C5a and by peptide EX-1 representing amino residues 1-31 on the N-terminal domain of the C5aR. In addition, FITC-labelled C5a bound to HMC-1, and this binding could be blocked by unlabelled C5a or C5aR antibodies. C5aR-specific mRNA was detected in HMC-1 cells by RT-PCR which confirmed the expression of the C5aR gene made by these cells. Lymphocyte-conditioned medium, interferon-γ or phorbol esters which have been shown to induce a down-regulation of C5aR On myeloid cells did not influence the expression of C5aR on HMC-1. C5a let to a transient mobilization of intracellular calcium in HMC-1 which could be inhibited by preincubation of C5a with a C5a-specific antibody. In contrast to findings with granulocytes, HMC-1 did not respond to C5a(desArg), confirming previous findings with human skin mast cells. The findings show that (i) although HMC-1 differ from granulocytes in their responsiveness to C5a(desArg), they express similar C5aR and (ii) HMC-1 resemble skin mast cells in the expression and function of C5aR and may therefore serve as a model in future studies addressing the biology of this anaphylatoxin receptor on skin mast cells.

AB - The expression of the receptor for the anaphylatoxin C5a (C5aR, CD88) on the human mast cell line HMC-1 was studied with four anti-C5aR monoclonal antibodies directed to the N-terminal domain of the receptor. All antibodies bound to the human mart cell line HMC-1. The binding could be blocked by recombinant C5a and by peptide EX-1 representing amino residues 1-31 on the N-terminal domain of the C5aR. In addition, FITC-labelled C5a bound to HMC-1, and this binding could be blocked by unlabelled C5a or C5aR antibodies. C5aR-specific mRNA was detected in HMC-1 cells by RT-PCR which confirmed the expression of the C5aR gene made by these cells. Lymphocyte-conditioned medium, interferon-γ or phorbol esters which have been shown to induce a down-regulation of C5aR On myeloid cells did not influence the expression of C5aR on HMC-1. C5a let to a transient mobilization of intracellular calcium in HMC-1 which could be inhibited by preincubation of C5a with a C5a-specific antibody. In contrast to findings with granulocytes, HMC-1 did not respond to C5a(desArg), confirming previous findings with human skin mast cells. The findings show that (i) although HMC-1 differ from granulocytes in their responsiveness to C5a(desArg), they express similar C5aR and (ii) HMC-1 resemble skin mast cells in the expression and function of C5aR and may therefore serve as a model in future studies addressing the biology of this anaphylatoxin receptor on skin mast cells.

UR - http://www.scopus.com/inward/record.url?scp=0029885592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029885592&partnerID=8YFLogxK

M3 - Article

C2 - 8693289

AN - SCOPUS:0029885592

VL - 44

SP - 30

EP - 36

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 1

ER -