The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg)

T. Werfel, M. Oppermann, J. H. Butterfield, G. Begemann, J. Elsner, O. Götze, J. Zwirner

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The expression of the receptor for the anaphylatoxin C5a (C5aR, CD88) on the human mast cell line HMC-1 was studied with four anti-C5aR monoclonal antibodies directed to the N-terminal domain of the receptor. All antibodies bound to the human mart cell line HMC-1. The binding could be blocked by recombinant C5a and by peptide EX-1 representing amino residues 1-31 on the N-terminal domain of the C5aR. In addition, FITC-labelled C5a bound to HMC-1, and this binding could be blocked by unlabelled C5a or C5aR antibodies. C5aR-specific mRNA was detected in HMC-1 cells by RT-PCR which confirmed the expression of the C5aR gene made by these cells. Lymphocyte-conditioned medium, interferon-γ or phorbol esters which have been shown to induce a down-regulation of C5aR On myeloid cells did not influence the expression of C5aR on HMC-1. C5a let to a transient mobilization of intracellular calcium in HMC-1 which could be inhibited by preincubation of C5a with a C5a-specific antibody. In contrast to findings with granulocytes, HMC-1 did not respond to C5a(desArg), confirming previous findings with human skin mast cells. The findings show that (i) although HMC-1 differ from granulocytes in their responsiveness to C5a(desArg), they express similar C5aR and (ii) HMC-1 resemble skin mast cells in the expression and function of C5aR and may therefore serve as a model in future studies addressing the biology of this anaphylatoxin receptor on skin mast cells.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalScandinavian Journal of Immunology
Volume44
Issue number1
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Immunology

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    Werfel, T., Oppermann, M., Butterfield, J. H., Begemann, G., Elsner, J., Götze, O., & Zwirner, J. (1996). The human mast cell line HMC-1 expresses c5a receptors and responds to C5a but not to C5(desArg). Scandinavian Journal of Immunology, 44(1), 30-36. https://doi.org/10.1046/j.1365-3083.1996.d01-272.x