The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells

P. A. Cattini, T. R. Anderson, J. D. Baxter, P. Mellon, N. L. Eberhardt

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Human growth hormone (hGH) gene expression and regulation by thyroid and glucocorticoid hormones were examined after its stable introduction into rat anterior pituitary tumor cells. Transcripts from the hGH gene were correctly initiated and could be positively regulated by glucocorticoids. In contrast to the endogenous rat GH gene and a transfected, modified rat GH gene, the hGH gene was negatively regulated by triiodothyronine. Analysis of hGH gene 5'-flanking deletions indicated that a distal regulatory element is required for efficient transcription. Thus promoter, glucocorticoid, and thyroid hormone control elements are present on the transfected hGH gene, whose expression may be negatively controlled by thyroid hormone.

Original languageEnglish (US)
Pages (from-to)13367-13372
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number28
StatePublished - 1986
Externally publishedYes

Fingerprint

Human Growth Hormone
Pituitary Neoplasms
Triiodothyronine
Rats
Tumors
Genes
Cells
Thyroid Hormones
Gene expression
Glucocorticoids
Gene Expression Regulation
Transcription
Hormones
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cattini, P. A., Anderson, T. R., Baxter, J. D., Mellon, P., & Eberhardt, N. L. (1986). The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells. Journal of Biological Chemistry, 261(28), 13367-13372.

The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells. / Cattini, P. A.; Anderson, T. R.; Baxter, J. D.; Mellon, P.; Eberhardt, N. L.

In: Journal of Biological Chemistry, Vol. 261, No. 28, 1986, p. 13367-13372.

Research output: Contribution to journalArticle

Cattini, PA, Anderson, TR, Baxter, JD, Mellon, P & Eberhardt, NL 1986, 'The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells', Journal of Biological Chemistry, vol. 261, no. 28, pp. 13367-13372.
Cattini, P. A. ; Anderson, T. R. ; Baxter, J. D. ; Mellon, P. ; Eberhardt, N. L. / The human growth hormone gene is negatively regulated by triiodothyronine when transfected into rat pituitary tumor cells. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 28. pp. 13367-13372.
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