The homeodomain transcription factor CDP/cut interacts with the cell cycle regulatory element of histone H4 genes packaged into nucleosomes

Thomas J. Last, André J. Van Wijnen, Marleen C. De Ridder, Gary S. Stein, Janet L. Stein

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The homeodomain transcription factor CDP/cut contains four separate DNA binding domains and interacts with large segments of DNA. Thus, CDP/cut has the potential to function as an architectural protein and perhaps to support modifications in chromatin structure and nucleosomal organization. To begin to examine the ability of CDP/cut to interact with chromatin. We analyzed binding of CDP/cut to the histone H4 gene promoter (-90 to +75) reconstituted into nucleosome cores. The -90 to +75 region encompasses the cell cycle regulatory element (Site II) that controls histone H4 gene transcription, a CDP/cut binding site and a nuclease hypersensitive region. Using electrophoretic mobility shift assays and DNase I footprinting experiments, we show that CDP/cut specifically interacts with its recognition motif in a nucleosomal context without displacing the nucleosome core. The competency of CDP/cut to interact with nucleosomes suggests that this transcription factor may facilitate chromatin remodeling in response to cell cycle regulatory and/or developmental cues.

Original languageEnglish (US)
Pages (from-to)185-194
Number of pages10
JournalMolecular Biology Reports
Volume26
Issue number3
DOIs
StatePublished - Aug 1 1999

Keywords

  • Cell cycle
  • Chromatin
  • Histone
  • Homeodomain
  • Nncleosome
  • Tanscription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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