The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met

Koji Yano, Kazuhiko Nakao, Koichi Sayama, Keisuke Hamasaki, Yuji Kato, Keisuke Nakata, Nobuko Ishii, Joseph H. Butterfield, Stephen J. Galli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) was originally characterized as a strong inducer of liver regeneration. However, it is now clear that HGF and its receptor, the proto-oncogene c-met, can be expressed in many other tissues, and that HGF can mediate diverse biological activities. We investigated the expression and function of c-met in a human mast cell line (HMC-1). We found that HMC-1 cells express c-met and that c-met expression can be upregulated by treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-α) in response to stimulation with PMA and the calcium ionophore, A23187. These results add the inhibition of TNF-α production to the other recognized effects of HGF/c-met on cellular function.

Original languageEnglish (US)
Pages (from-to)740-745
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number3
DOIs
StatePublished - Oct 29 1997

Fingerprint

Proto-Oncogene Proteins c-met
Hepatocyte Growth Factor
Mast Cells
Cells
Cell Line
Acetates
Tumor Necrosis Factor-alpha
Liver Regeneration
Proto-Oncogenes
Calcium Ionophores
Calcimycin
Bioactivity
Liver
Tissue

Keywords

  • c-met proto-oncogene
  • Mast cell
  • Tumor necrosis factor-alpha

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met. / Yano, Koji; Nakao, Kazuhiko; Sayama, Koichi; Hamasaki, Keisuke; Kato, Yuji; Nakata, Keisuke; Ishii, Nobuko; Butterfield, Joseph H.; Galli, Stephen J.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 3, 29.10.1997, p. 740-745.

Research output: Contribution to journalArticle

Yano, K, Nakao, K, Sayama, K, Hamasaki, K, Kato, Y, Nakata, K, Ishii, N, Butterfield, JH & Galli, SJ 1997, 'The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met', Biochemical and Biophysical Research Communications, vol. 239, no. 3, pp. 740-745. https://doi.org/10.1006/bbrc.1997.7546
Yano, Koji ; Nakao, Kazuhiko ; Sayama, Koichi ; Hamasaki, Keisuke ; Kato, Yuji ; Nakata, Keisuke ; Ishii, Nobuko ; Butterfield, Joseph H. ; Galli, Stephen J. / The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 239, No. 3. pp. 740-745.
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