The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met

Koji Yano, Kazuhiko Nakao, Koichi Sayama, Keisuke Hamasaki, Yuji Kato, Keisuke Nakata, Nobuko Ishii, Joseph H. Butterfield, Stephen J. Galli

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Hepatocyte growth factor (HGF) was originally characterized as a strong inducer of liver regeneration. However, it is now clear that HGF and its receptor, the proto-oncogene c-met, can be expressed in many other tissues, and that HGF can mediate diverse biological activities. We investigated the expression and function of c-met in a human mast cell line (HMC-1). We found that HMC-1 cells express c-met and that c-met expression can be upregulated by treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-α) in response to stimulation with PMA and the calcium ionophore, A23187. These results add the inhibition of TNF-α production to the other recognized effects of HGF/c-met on cellular function.

Original languageEnglish (US)
Pages (from-to)740-745
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number3
DOIs
StatePublished - Oct 29 1997

Keywords

  • Mast cell
  • Tumor necrosis factor-alpha
  • c-met proto-oncogene

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Yano, K., Nakao, K., Sayama, K., Hamasaki, K., Kato, Y., Nakata, K., Ishii, N., Butterfield, J. H., & Galli, S. J. (1997). The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met. Biochemical and Biophysical Research Communications, 239(3), 740-745. https://doi.org/10.1006/bbrc.1997.7546