TY - JOUR
T1 - The HMC-1 human mast cell line expresses the hepatocyte growth factor receptor c-met
AU - Yano, Koji
AU - Nakao, Kazuhiko
AU - Sayama, Koichi
AU - Hamasaki, Keisuke
AU - Kato, Yuji
AU - Nakata, Keisuke
AU - Ishii, Nobuko
AU - Butterfield, Joseph H.
AU - Galli, Stephen J.
N1 - Funding Information:
This work was supported in part by United States Public Health Services Grant P50 HL-56383, Project 4 (S. J. Galli).
PY - 1997/10/29
Y1 - 1997/10/29
N2 - Hepatocyte growth factor (HGF) was originally characterized as a strong inducer of liver regeneration. However, it is now clear that HGF and its receptor, the proto-oncogene c-met, can be expressed in many other tissues, and that HGF can mediate diverse biological activities. We investigated the expression and function of c-met in a human mast cell line (HMC-1). We found that HMC-1 cells express c-met and that c-met expression can be upregulated by treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-α) in response to stimulation with PMA and the calcium ionophore, A23187. These results add the inhibition of TNF-α production to the other recognized effects of HGF/c-met on cellular function.
AB - Hepatocyte growth factor (HGF) was originally characterized as a strong inducer of liver regeneration. However, it is now clear that HGF and its receptor, the proto-oncogene c-met, can be expressed in many other tissues, and that HGF can mediate diverse biological activities. We investigated the expression and function of c-met in a human mast cell line (HMC-1). We found that HMC-1 cells express c-met and that c-met expression can be upregulated by treatment of the cells with phorbol 12-myristate 13-acetate (PMA). Although HGF did not detectably influence the proliferation or morphology of HMC-1 cells, HGF inhibited the cells' ability to release tumor necrosis factor-alpha (TNF-α) in response to stimulation with PMA and the calcium ionophore, A23187. These results add the inhibition of TNF-α production to the other recognized effects of HGF/c-met on cellular function.
KW - Mast cell
KW - Tumor necrosis factor-alpha
KW - c-met proto-oncogene
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U2 - 10.1006/bbrc.1997.7546
DO - 10.1006/bbrc.1997.7546
M3 - Article
C2 - 9367839
AN - SCOPUS:0031590426
SN - 0006-291X
VL - 239
SP - 740
EP - 745
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -