The histone gene transcription factor HiNF-P stabilizes its cell cycle regulatory co-activator p220NPAT

Ricardo Medina, Andre J. Van Wijnen, Gary S. Stein, Janet L. Stein

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Orderly progression through the cell cycle requires the transcriptional activation of histone genes to support packaging of newly replicated DNA. Induction of human histone gene expression is mediated by a co-activation complex containing transcription factor HiNF-P and its cofactor p220 NPAT. Here, using cells synchronized in S-phase and in mitosis, as well as serum-stimulated cells, we have investigated how HiNF-P is regulated during the cell cycle and examined its stability relative to p220 NPAT. We find that while HiNF-P is maintained at steady-state levels throughout the cell cycle, both HiNF-P and p220NPAT are actively degraded by the proteasome pathway. Importantly, elevation of HiNF-P levels enhances the stability of its co-activator p220NPAT. The HiNF-P-dependent stabilization of p220NPAT may reinforce signaling through the cyclin E/CDK2/p220NPAT pathway and contribute to coordinate control of histone gene expression.

Original languageEnglish (US)
Pages (from-to)15915-15920
Number of pages6
JournalBiochemistry
Volume45
Issue number51
DOIs
StatePublished - Dec 26 2006

ASJC Scopus subject areas

  • Biochemistry

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