The histologic effect of barrier vein wrapping of peripheral nerves

David S. Ruch, Robert M. Spinner, L. Andrew Koman, Venkata R. Challa, Dermot O'Farrell, L. Scott Levin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Twenty-four Sprague-Dawley rats underwent exploration and exposure of the sciatic nerve, followed by sham isolation, irrigation, and subsequent wound closure (Group 1); barrier vein wrapping of the nerve using glutaraldehyde-preserved allograft inferior vena cava (Group 2); or barrier vein wrapping of the nerve with femoral vein autograft from the contralateral extremity (Group 3). Four months later, the rats were sacrificed and the nerves fixed in situ in buffered glutaraldehyde. Sections proximal, mid- portion, and distal to the barrier and vein wrap were fixed with osmium tetroxide, epon embedded, stained with toluidine blue, and studied under x200 and x400 light microscopy. Epineural scar formation increased 10 times in Group 2, compared to Group 3 (p < 0.0001). Epineural thickness and the number of degenerating axons did not differ significantly among all groups. Inflammatory cells in Group 2 increased 100 times, compared to Group 3 (p < 0.0001). Continuity with the underlying epineural layer was apparent grossly and microscopically with all allografts, but with no autografts. Thus glutaraldehyde-preserved allograft vein wraps appear to incite a marked inflammatory response, with epineural scarring and adherence to the underlying nerve, while autograft vein wraps do not.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalJournal of Reconstructive Microsurgery
Volume12
Issue number5
StatePublished - Jul 1996
Externally publishedYes

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Peripheral Nerves
Veins
Autografts
Glutaral
Allografts
Cicatrix
Osmium Tetroxide
Tolonium Chloride
Femoral Vein
Inferior Vena Cava
Sciatic Nerve
Sprague Dawley Rats
Axons
Microscopy
Extremities
Light
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Ruch, D. S., Spinner, R. M., Koman, L. A., Challa, V. R., O'Farrell, D., & Levin, L. S. (1996). The histologic effect of barrier vein wrapping of peripheral nerves. Journal of Reconstructive Microsurgery, 12(5), 291-296.

The histologic effect of barrier vein wrapping of peripheral nerves. / Ruch, David S.; Spinner, Robert M.; Koman, L. Andrew; Challa, Venkata R.; O'Farrell, Dermot; Levin, L. Scott.

In: Journal of Reconstructive Microsurgery, Vol. 12, No. 5, 07.1996, p. 291-296.

Research output: Contribution to journalArticle

Ruch, DS, Spinner, RM, Koman, LA, Challa, VR, O'Farrell, D & Levin, LS 1996, 'The histologic effect of barrier vein wrapping of peripheral nerves', Journal of Reconstructive Microsurgery, vol. 12, no. 5, pp. 291-296.
Ruch DS, Spinner RM, Koman LA, Challa VR, O'Farrell D, Levin LS. The histologic effect of barrier vein wrapping of peripheral nerves. Journal of Reconstructive Microsurgery. 1996 Jul;12(5):291-296.
Ruch, David S. ; Spinner, Robert M. ; Koman, L. Andrew ; Challa, Venkata R. ; O'Farrell, Dermot ; Levin, L. Scott. / The histologic effect of barrier vein wrapping of peripheral nerves. In: Journal of Reconstructive Microsurgery. 1996 ; Vol. 12, No. 5. pp. 291-296.
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