The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes

Ricardo Medina, Margaretha Van Der Deen, Angela Miele-Chamberland, Rong Lin Xie, Andre J van Wijnen, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

HiNF-P and its cofactor p220NPAT are principal factors regulating histone gene expression at the G1-S phase cell cycle transition. Here, we have investigated whether HiNF-P controls other cell cycle- and cancer-related genes. We used cDNA microarrays to monitor responsiveness of gene expression to small interfering RNA-mediated depletion of HiNF-P. Candidate HiNF-P target genes were examined for the presence of HiNF-P recognition motifs, in vitro HiNF-P binding to DNA,and in vivo association by chromatin immunoprecipitations and functional reporter gene assays. Of 177 proliferation-related genes we tested, 20 are modulated in HiNF-P-depleted cells and contain putative HiNF-P binding motifs. We validated that at least three genes (i.e., ATM, PRKDC, and CKS2) are HiNF-P dependent and provide data indicating that the DNA damage response is altered in HiNF-P -depleted cells. We conclude that,in addition to histone genes, HiNF-P also regulates expression of nonhistone targets that influence competency for cell cycle progression.

Original languageEnglish (US)
Pages (from-to)10334-10342
Number of pages9
JournalCancer Research
Volume67
Issue number21
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Cell Cycle
Histones
Genes
Gene Expression
Chromatin Immunoprecipitation
Neoplasm Genes
G1 Phase
Cell Cycle Checkpoints
Oligonucleotide Array Sequence Analysis
S Phase
Reporter Genes
Small Interfering RNA
DNA Damage
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Medina, R., Van Der Deen, M., Miele-Chamberland, A., Xie, R. L., van Wijnen, A. J., Stein, J. L., & Stein, G. S. (2007). The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes. Cancer Research, 67(21), 10334-10342. https://doi.org/10.1158/0008-5472.CAN-07-1560

The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes. / Medina, Ricardo; Van Der Deen, Margaretha; Miele-Chamberland, Angela; Xie, Rong Lin; van Wijnen, Andre J; Stein, Janet L.; Stein, Gary S.

In: Cancer Research, Vol. 67, No. 21, 01.11.2007, p. 10334-10342.

Research output: Contribution to journalArticle

Medina, R, Van Der Deen, M, Miele-Chamberland, A, Xie, RL, van Wijnen, AJ, Stein, JL & Stein, GS 2007, 'The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes', Cancer Research, vol. 67, no. 21, pp. 10334-10342. https://doi.org/10.1158/0008-5472.CAN-07-1560
Medina R, Van Der Deen M, Miele-Chamberland A, Xie RL, van Wijnen AJ, Stein JL et al. The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes. Cancer Research. 2007 Nov 1;67(21):10334-10342. https://doi.org/10.1158/0008-5472.CAN-07-1560
Medina, Ricardo ; Van Der Deen, Margaretha ; Miele-Chamberland, Angela ; Xie, Rong Lin ; van Wijnen, Andre J ; Stein, Janet L. ; Stein, Gary S. / The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes. In: Cancer Research. 2007 ; Vol. 67, No. 21. pp. 10334-10342.
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