THE HAEM‐ACCESSIBILITY IN LEGHAEMOGLOBIN OF LUPINUS LUTEUS AS OBSERVED BY PROTON MAGNETIC RELAXATION

S. Vuk‐Pavlović; B. Benko; S. Maričic; G. Lahajnar; I. P. Kuranova; B. K. Vainshtein

doi:10.1111/j.1399-3011.1976.tb02522.x

THE HAEM‐ACCESSIBILITY IN LEGHAEMOGLOBIN OF LUPINUS LUTEUS AS OBSERVED BY PROTON MAGNETIC RELAXATION

S. Vuk‐Pavlović, B. Benko, S. Maričic, G. Lahajnar, I. P. Kuranova, B. K. Vainshtein

Hematology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Using the solvent‐protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our “stereo‐chemical p.m.r. titration” method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemoglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHz. Taking into account the measured value of τ_c = (7.7 ± 0.5) × 10^‐10 s the iron‐to‐proton inter‐spin distances have been calculated. The significance of these distances as well as the electronic g‐factor anisotropy for elucidation of fine structural details of the haem‐environment are discussed.

Original language	English (US)
Pages (from-to)	427-434
Number of pages	8
Journal	International Journal of Peptide and Protein Research
Volume	8
Issue number	5
DOIs	https://doi.org/10.1111/j.1399-3011.1976.tb02522.x
State	Published - Sep 1976

ASJC Scopus subject areas

Biochemistry

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title = "THE HAEM‐ACCESSIBILITY IN LEGHAEMOGLOBIN OF LUPINUS LUTEUS AS OBSERVED BY PROTON MAGNETIC RELAXATION",

abstract = "Using the solvent‐protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our “stereo‐chemical p.m.r. titration” method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemoglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHz. Taking into account the measured value of τc = (7.7 ± 0.5) × 10‐10 s the iron‐to‐proton inter‐spin distances have been calculated. The significance of these distances as well as the electronic g‐factor anisotropy for elucidation of fine structural details of the haem‐environment are discussed.",

author = "S. Vuk‐Pavlovi{\'c} and B. Benko and S. Mari{\v c}ic and G. Lahajnar and Kuranova, {I. P.} and Vainshtein, {B. K.}",

year = "1976",

month = sep,

doi = "10.1111/j.1399-3011.1976.tb02522.x",

language = "English (US)",

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AU - Vuk‐Pavlović, S.

AU - Benko, B.

AU - Maričic, S.

AU - Lahajnar, G.

AU - Kuranova, I. P.

AU - Vainshtein, B. K.

PY - 1976/9

Y1 - 1976/9

N2 - Using the solvent‐protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our “stereo‐chemical p.m.r. titration” method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemoglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHz. Taking into account the measured value of τc = (7.7 ± 0.5) × 10‐10 s the iron‐to‐proton inter‐spin distances have been calculated. The significance of these distances as well as the electronic g‐factor anisotropy for elucidation of fine structural details of the haem‐environment are discussed.

AB - Using the solvent‐protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our “stereo‐chemical p.m.r. titration” method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemoglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHz. Taking into account the measured value of τc = (7.7 ± 0.5) × 10‐10 s the iron‐to‐proton inter‐spin distances have been calculated. The significance of these distances as well as the electronic g‐factor anisotropy for elucidation of fine structural details of the haem‐environment are discussed.

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THE HAEM‐ACCESSIBILITY IN LEGHAEMOGLOBIN OF LUPINUS LUTEUS AS OBSERVED BY PROTON MAGNETIC RELAXATION

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