The growth and phenotypic expression of human osteoblasts

C. R. Ruder, P. Dixon, A. G. Mikos, M. J. Yaszemski

Research output: Contribution to journalArticle

Abstract

The care of patients with a skeletal deficiency currently involves the use of bone graft or a non-biologic material such as a metal or polymer. There are alternate possibilities in development which involve the growth of bone cells (osteoblasts) on degradable polymer scaffolds. These tissue engineering strategies require production of the polymeric scaffold, cellular harvest followed by either ex vivo or in vivo growth of the cells on the scaffold, and exploration of the interaction between the cell and scaffold. Research into these strategies utilizes cells from a variety of species, but clinical applications will likely require human osteoblasts. This study explores the process whereby human osteoblasts are harvested under sterile conditions during joint replacement surgery from normally discarded cancellous bone, transported from the operating room to the lab, and grown in culture. This process is feasible, and the cells express their phenotype via the production of alkaline phosphatase and collagen in culture.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalCytotechnology
Volume22
Issue number1-3
DOIs
StatePublished - Jan 1 1996

Keywords

  • biodegradable
  • bone regeneration
  • cell culture
  • human cell osteoblasts
  • polymers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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