The G protein βγ subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes

Lisa Stehno-Bittel, Grigory Krapivinsky, Lyubov Krapivinsky, Carmen M Terzic, David E. Clapham

Research output: Contribution to journalArticle

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Abstract

At least 30 G protein-linked receptors stimulate phosphatidylinositol 4,5-bisphosphate phosphodiesterase (phospholipase Cβ, PLCβ) through G protein subunits to release intracellular calcium from the endoplasmic reticulum (Clapham, D. E. (1995) Cell 80, 259-268). Although both Gα and Gβγ G protein subunits have been shown to activate purified PLCβ in vitro, Gαq has been presumed to mediate the pertussis toxin-insensitive response in vivo. In this study, we show that Gβγ plays a dominant role in muscarinic-mediated activation of PLCβ by employing the Xenopus oocyte expression system. Antisense nucleotides and antibodies to Gαq/11 blocked the m3-mediated signal transduction by inhibiting interaction of the muscarinic receptor with the G protein. Agents that specifically bound free Gβγ subunits (Gα-GDP and a β-adrenergic receptor kinase fragment) inhibited acetylcholine-induced signal transduction to PLCβ, and injection of Gβγ subunits into oocytes directly induced release of intracellular Ca2+. We conclude that receptor coupling specificity of the Gαq/Gβγ heterotrimer is determined by Gαq; Gβγ is the predominant signaling molecule activating oocyte PLCβ.

Original languageEnglish (US)
Pages (from-to)30068-30074
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number50
StatePublished - Dec 15 1995

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Protein Subunits
Type C Phospholipases
Muscarinic Receptors
Xenopus
GTP-Binding Proteins
Oocytes
Signal transduction
Signal Transduction
Type 5 Cyclic Nucleotide Phosphodiesterases
Pertussis Toxin
Phosphoric Diester Hydrolases
Endoplasmic Reticulum
Adrenergic Receptors
Cholinergic Agents
Acetylcholine
Phosphotransferases
Nucleotides
Chemical activation
Calcium
Injections

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stehno-Bittel, L., Krapivinsky, G., Krapivinsky, L., Terzic, C. M., & Clapham, D. E. (1995). The G protein βγ subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes. Journal of Biological Chemistry, 270(50), 30068-30074.

The G protein βγ subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes. / Stehno-Bittel, Lisa; Krapivinsky, Grigory; Krapivinsky, Lyubov; Terzic, Carmen M; Clapham, David E.

In: Journal of Biological Chemistry, Vol. 270, No. 50, 15.12.1995, p. 30068-30074.

Research output: Contribution to journalArticle

Stehno-Bittel, L, Krapivinsky, G, Krapivinsky, L, Terzic, CM & Clapham, DE 1995, 'The G protein βγ subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes', Journal of Biological Chemistry, vol. 270, no. 50, pp. 30068-30074.
Stehno-Bittel, Lisa ; Krapivinsky, Grigory ; Krapivinsky, Lyubov ; Terzic, Carmen M ; Clapham, David E. / The G protein βγ subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 50. pp. 30068-30074.
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